益心脑滴丸对血小板聚集、血栓形成及凝血作用的影响

目的: 研究益心脑滴丸对血小板聚集、血栓形成及凝血作用的影响。 方法: 将Wistar大鼠随机分为5组,分别为正常组、阿司匹林组、益心脑滴丸高、中、低剂量组(906,453,226 mg·kg^-1,ig)。分别由ADP、凝血酶、CaCl₂诱导血小板聚集,采用比浊法观察益心脑滴丸对大鼠血小板聚集率的影响;通过毛细玻璃管法研究益心脑滴丸对血瘀模型大鼠凝血时间的影响,同时评价滴丸对大鼠凝血酶原时间(PT)及凝血酶时间(TT)的作用;采用大鼠动静脉旁路血栓形成法,检测益心脑滴丸抗血栓的作用。 结果: 益心脑滴丸各剂量组均可明显抑制ADP、凝血酶及CaCl₂诱导的大鼠血小板最大聚集率(P<0.05或P<0.01);能够不同程度延长大鼠 PT,TT及凝血时间(P<0.05或P<0.01);益心脑滴丸高、中剂量能够抑制大鼠血栓形成,减轻血栓质量(P<0.05或P<0.01)。 结论: 益心脑滴丸可以对抗血栓形成,具有抑制血小板聚集及抗凝血的作用。

Effects of Yixinnao Dripping Pills on Platelet Aggregation and Thrombus Formation and Coagulability Activity

Objective: To investigate the effects of Yixinnao dripping pills on platelet aggregation and thrombus formation in rats, as well as the effects on coagulability activity. Method: The Wistar rats were divided into normal control group, aspirin group, Yixinnao dripping pills high, middle and low dose group(906, 453, 226 mg·kg^-1, ig, accordingly). The effects of Yixinnao dripping pills on platelet aggregation in vivo were measured by the model induced by ADP, thrombin and CaCl₂.Coagulation time in acute blood stasis rat models was determined by the glass capillary method and the effects were also evaluated on prothrombin time (PT) and thrombin time (TT) in these groups. The inhibitory action of Yixinnao dripping pills on the thrombus formation was investigated by the model of artery-vein bypass thrombosis. Result: Yixinnao dripping pills in the high, middle and low dose could signifiinhibit platelet aggregation induced by ADP, thrombin and CaCl₂, respectly (P<0.05 or P<0.01). It could also lengthen coagulation time, TT and PT in different degree (P<0.05 or P<0.01).Moreover, Yixinnao dripping pills (906, 453 mg·kg^-1) could markedly restrain thrombus formation, reduce thrombus weight (P<0.05 or P<0.01). Conclusion: Yixinnao dripping pills showed the function of anti-thrombosis, and exerted the effects on inhibition of platelet aggregation and coagulability.