均匀设计法优选双藤痹痛凝胶膏剂的基质处方

目的：分析女贞子酒蒸过程中3种裂环环烯醚萜苷类成分的动态变化规律。方法：采用HPLC同时测定女贞子生品及酒蒸不同时间（4，8，12，16，20，24 h）炮制品中新女贞苷、木樨榄苷-11-甲酯和女贞酸含量，流动相乙腈（A）-0.1%甲酸溶液（B）梯度洗脱（0~19 min，12%~25%A），检测波长235 nm。结果：新女贞苷、木樨榄苷-11-甲酯、女贞酸的线性范围分别为0.034 5~3.450，0.005 86~0.586，0.028 6~2.860 μg，平均加样回收率分别为99.49%，99.02%，97.80%。女贞子酒蒸后新女贞苷、木樨榄苷-11-甲酯含量显著降低，而女贞酸含量显著升高；在24 h内，随着蒸制时间的延长，新女贞苷、木樨榄苷-11-甲酯含量逐渐降低，而女贞酸含量逐渐升高后达稳定状态。结论：女贞子酒蒸后新女贞苷、木樨榄苷-11-甲酯产生质变，女贞酸发生量变，为科学阐释女贞子炮制机制及其饮片的规范化管理提供实验依据。

Optimization of Matrix Formulation of Shuangteng Bitong Gel Ointments by Uniform Design

Objective: To optimize matrix formulation of Shuangteng Bitong gel ointments. Method: Taking initial adhesion,peeling strength and overall sensory score as comprehensive evaluation indexes,sodium polyacrylate(NP700) as framework material,kaolin as filler,carbomer 940,polyvinyl alcohol(PVA-124) and sodium carboxymethyl cellulose(CMC-Na) as thickening agent,glycerin as humectant,dihydroxyaluminum aminoacetate as crosslinking agent,citric acid as crossliked regulator,castor oil as softener,matrix formulation of Shuangteng Bitong gel ointments was optimized by uniform design. Result: Optimum matrix formulation was as following:NP700:carbomer 940:PVA-124:CMC-Na:kaolin:glycerin:dihydroxyaluminum aminoacetate:citric acid:castor oil=1.68:0.43:0.1:0.33:1.08:12.0:0.09:0.14:0.05;initial adhesion was 12.1 mm,peeling strength was 34.56 s,overall sensory score was 27.7. Conclusion: These prepared Shuangteng Bitong gel ointments had smooth appearance and no film residue with good adhesive property and good skin adhesive ability.