附子理中汤对脾阳虚证大鼠血糖、甘油三酯及总胆固醇的影响

目的:分析附子理中汤对脾阳虚证大鼠血糖、甘油三酯(TG)以及总胆固醇(TC)水平的影响和内在联系.方法:大鼠共4组,每组16只.①对照组:普通饲料喂养,自由饮水；②阳虚组:手术切除肩胛骨间棕色脂肪组织( brown adipose tissue,BAT),余同①组；③脾阳虚组:高脂饲料(83％普通饲料,15％ TG(TG),2％(TC)在19℃环境喂养,余同②组；④中药组:附子理中汤每天按4 g·kg-1ig;余同③组.全部4组动物4周后第1天左颈总动脉插管取血检测血糖、血清甘油三酯(TG),血清总胆固醇(TC)含量.结果:①各组血糖含量(mmol·L -)的比较:阳虚组(1.40±0.071)与对照组(5.41±0.08)相比血糖降低(P＜0.01)；脾阳虚组(5.81±0.08)与阳虚组相比血糖升高(P＜0.01)；附子理中汤组(5.01±0.08)与阳虚组相比,血糖升高(P＜0.01)；附子理中汤组与脾阳虚组相比,血糖有下降趋势.②各组血清TG含量(mmol·L-1)的比较:阳虚组(0.284±0.110)与对照组(0.487±0.076)相比血清TG降低(P＜0.01)；脾阳虚组(0.620±0.184)与阳虚组相比血清TG增加(P＜0.01)；附子理中汤组(0.462±0.111)与阳虚组相比,血清TG升高(P＜0.01),附子理中汤组与脾阳虚组相比,血清TG有下降趋势.③各组血清TC含量(mmol·L -)的比较:脾阳虚组(0.183±0.025)、附子理中汤组(0.211±0.026)与对照组(0.081±0.020)相比血清总TC均升高(P＜0.01).结论:附子理中汤使血清TG降低；增加对葡萄糖的利用及加强其代谢,使血糖水平降低.附子理中汤“健脾”功效表现为促使糖和脂类代谢趋向正常,从而使血糖和血脂向恢复正常水平的方向转化.

Influences of Fuzi Lizhong decoction on Blood Sugar, Triglyceride and Total Cholesterol in Rats with Spleen Yang Deficiency Syndrome

Objective:To explore influences of Fuzi Lizhong decoction on blood sugar,triglyceride and total cholesterol in rats with spleen yang deficiency syndrome.Method: The rats were randomly divided into four groups,16 cases each group ①control group: fed with ordinary food and water.②yang deficiency syndrome group: the same as the control group’ except removing brown adipose tissue.③spleen yang deficiency syndrome group: the same as the yang deficiency syndrome group except that fed with high fatty diet(including 83% ordinary diet,15% triglycerides,2% cholesterol) and placed in circumstance of 19 ℃.④Chinese herbal decoction group: the same as the spleen yang deficiency syndrome group’ except ig 4 g·kg-1 each day with Fuzi Lizhong decoction.On the first day after 4 weeks blood from left common carotid artery was collected in all the rats in the four groups and then to test the content of blood sugar,triglyceride,total cholesterol.Result: ① Content of blood sugar: compared with control group(5.41±0.080)mmol·L-1,content of blood sugar was lower than that in yang deficiency syndrome group(1.40±0.071) mmol·L-1(P<0.01);compared with yang deficiency syndrome group,content of blood sugar in spleen yang deficiency syndrome group(5.81±0.081) mmol·L-1 was higher(P<0.01);compared with yang deficiency syndrome group,content of blood sugar in Chinese herbal decoction group(5.01±0.080) was higher(P<0.01).② Content of triglyceride: compared with control group(0.487±0.076)mmol·L-1,content of triglyceride was lower than that in yang deficiency syndrome group(0.284±0.11) mmol·L-1(P<0.01);compared with yang deficiency syndrome group,content of triglyceride in spleen yang deficiency syndrome group(0.62±0.184) mmol·L-1 was higher(P<0.01);compared with yang deficiency syndrome group,content of triglyceride in Chinese herbal decoction group(0.462±0.111) mmol·L-1 was higher(P<0.01).③ Content of total cholesterol compared with control group(0.081±0.020)mmol·L-1,content of total cholesterol in both spleen yang deficiency syndrome group(0.183±0.025) mmol·L-1 and Chinese herbal decoction group(0.211±0.026) mmol·L-1 was higher(P<0.01).Conclusion: Fuzi Lizhong decoction can decrease the content of triglyceride and increase use of glucose and its metabolism and then causes its levels lower.Fuzi Lizhong decoction embodies its effect of refreshing spleen in traditional Chinese medicine by recovering the normal metabolism of blood sugar and lipids.