蚁龙通痹汤改善兔膝骨关节炎软骨组织骨生物力学的炎症机制

目的:观察蚁龙通痹汤对兔膝骨关节炎病理形态的影响,探讨其治疗骨关节炎可能的作用机制.方法:将40只日本雄性大耳白兔按体重分为4组,即正常组,模型组、蚁龙通痹汤组、葡立胶囊组,每组10只.除正常组外,其余各组均行右侧膝关节前交叉韧带切断建立骨关节炎病变模型.造模后第2天开始给药(葡立胶囊0.1 g·kg-1,蚁龙通痹汤生药0.6g·kg -,ig每日1次),连续4周.观察各组兔膝关节软骨及滑膜组织病理形态学改变,测定关节液中肿瘤坏死因子(TNF)-α和白介素(IL)-1β含量以及软骨下骨生物力学检测,并分析软骨.中基质金属蛋自酶(MMP)-1、组织金属蛋白酶抑制因子(TIMP-1)的表达情况.结果:与模型组比较,蚁龙通痹汤组动物关节液IL-1β和.TNF-α含量、软骨MMP-1和TIMP-1表达率、关节外在表现和软骨病理组织学变化,以及软骨最大载荷和弹性模量均出现明显差异改善.结论:蚁龙通痹汤能够减少兔膝骨关节炎关节液IL-1β和TNF-α的分泌,改善MMP-1和TIMP-1在软骨组织细胞中表达的平衡而达到对软骨组织保护的作用.

Inflammatory Mechanism of Anti-earthworm Tongbi-complex Decoction on Bone Biomechanics of Cartilaginous Tissue on the Rabbit with Knee Osteoarthritis

Objective: To observe the improvement effect of Anti-earthworm Tongbi-complex decoction on bone biomechanics of cartilaginous tissue on the rabbit models of knee osteoarthritis(KOA),and elucidate its potential mechanism.Methods: Forty healthy male rabbits were randomly divided into four groups: normal control group,model group,Anti-earthworm Tongbi-complex decoction group and glucosamine hydrochloride capsules group.All rabbits were prepared for right knee osteoarthritis model induced by anterior cruciate ligament transaction except the normal control group.The model rabbits were treated with Anti-earthworm Tongbi-complex decoction(0.6 g·kg-1,ig) and glucosamine hydrochloride Capsules(0.1 g·kg-1,ig) for 4 weeks.The following indexes was observed in all rabbits at experimental endpoint like the ultrastructure changes of arthrodial cartilage and synovium,levels of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) in synovial fluid by ELISIA,and the expression of matrix metallo-proteinase(MMP-1) and tissue inhibitor of tissue inhibitor of metalloproteinase-1(TIMP-1) by immunohistochemistry in cartilage tissue.Meanwhile,biomechanical test was performed to measure the maximal loading and compression modulus.Results: Compared with model group of OA,the indexes showed significant improvement in rabbits treated with Anti-earthworm Tongbi-complex decoction and glucosamine hydrochloride capsules group like the ultrastructure changes of arthrodial cartilage and synovium,levels of IL-1β and TNF-α in synovial fluid,the expression of MMP-1 and TIMP-1 in cartilage tissue,and biomechanical characters including the maximal loading and compression modulus.Conclusion: Ant-earthworm tongbi-complex decoction can decrease the levels of IL-1β and TNF-α in synovial fluid,improve the unbalancing expression of MMP-1 and TIMP-1 in articular chondrocytes,so as to protect cartilaginous tissue in the rabbit with knee osteoarthritis.