逍遥散对雷公藤致大鼠急性肝损伤的保护作用

目的:观察中医经典方剂逍遥散对雷公藤水煎剂致大鼠急性肝损伤的保护作用,并初步探讨相关机制.方法:60只大鼠随机分为空白对照组、模型组、甘利欣(0.056 g?kg-1)对照组、逍遥散低、中、高剂量组(3.38,6.75,13.50 g?kg-1).分别以相应剂量药物连续ig5 d,再以雷公藤水煎剂按生药量计3.2 mg?kg-1 ig 4 d造模.测定血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)含量,并做肝脏病理学检查.结果:与空白组比较,模型组血清ALT,AST及MDA水平明显升高(P＜0.05),SOD,GSH-Px水平明显降低(P＜0.05)；肝脏病理呈脂肪性病变,肝细胞浑浊.与模型组比较,逍遥散各组均可有效降低血清ALT,AST及MDA水平(P＜0.05),提高GSH-Px水平(P＜0.05),中、高剂量还可提高SOD水平(P＜0.05)；逍遥散各组肝脏病理损伤与模型组比较均有所减轻.结论:雷公藤所致急性肝损伤与其引起脂质过氧化反应有关；逍遥散对雷公藤所致急性肝损伤具有保护作用,其作用机制与抑制脂质过氧化反应有关.

Study on Protective Effect and Mechanism of Xiaoyao San on the Acute Liver Injury Induced by Tripterygium wilfordii

Objective:To observe the protective effect and mechanism of Xiaoyao San on rats’ acute liver injury induced by the decoction of Tripterygium wilfordii.Method: Sixty rats were randomly divided into control group,T.wilfordii model group,ganlixin group,low-dose,medium-dose and high-dose groups of Xiaoyao San.Each group was respectively fed with the corresponding dose of drug for 5 days.Then fed with decoction of T.Wilfordii for 4 days to make the model.alamine aminotransferase(ALT),aspartate aminotransferase(AST),superoxide dismutase(SOD),malondialdehyde(MDA) and glutathione peroxidase GSH-Px in serum were measured and liver pathology is detected.Result: Compared with the control group,the serum ALT,AST and MDA of model group were significantly increased,SOD and GSH-Px decreased obviously(P<0.05).Pathological investigation showed liver fatty change and turbid degeneration.Compared with model group,all doses of Xiaoyao San could effectively decrease ALT,AST and MDA,increase GSH-Px(P<0.05),medium-dose and high-dose also increase SOD(P<0.05),the liver injury was alleviated.Conclusion: T.wilfordii-induced acute liver injury is associated with lipid peroxidation.Xiaoyao San has effect of protecting the liver injury induced by T.wilfordii and the mechanism has a relationship with inhibition of lipid peroxidation.