| 蒲公英叶脂溶性成分的GC-MS分析及其活性探讨 |
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| 引用本文： | 段红波,宦臣臣,梁引库,徐仲阳,任晓峰.蒲公英叶脂溶性成分的GC-MS分析及其活性探讨[J].中国实验方剂学杂志,2018,24(1):60-66. |
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| 作者姓名： | 段红波 宦臣臣 梁引库 徐仲阳 任晓峰 |
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| 作者单位： | 陕西理工大学 陕西省资源生物重点实验室, 陕西 汉中 723000;陕西理工大学 生物科学与工程学院, 陕西 汉中 723000,陕西理工大学 陕西省资源生物重点实验室, 陕西 汉中 723000;陕西理工大学 生物科学与工程学院, 陕西 汉中 723000,陕西理工大学 陕西省资源生物重点实验室, 陕西 汉中 723000;陕西理工大学 生物科学与工程学院, 陕西 汉中 723000,青海大学 农林科学院, 西宁 810016,汉中天然谷生物科技股份有限公司, 陕西 汉中 723000 |
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| 基金项目： | 陕西省科技厅陕西省资源生物重点实验室项目（SZS-15-03）；陕西省科技厅社会发展项目（2014K13-23）；秦巴山区生物资源综合开发协同创新中心项目（QBXT-Z（Y）-15-2） |
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| 摘 要： | 目的:研究蒲公英叶脂溶性成分的组成及其抗氧化活性和抑菌活性。方法:利用索氏提取法提取蒲公英叶中的脂溶性成分,采用气相色谱质谱联用法(GC-MS)对其组成进行分析鉴定,分别采用磷钼络合物法、邻苯三酚自氧化分光光度法和Fenton氧化法测定其抗氧化活性,纸片抑菌法和微孔扩散法测定其抑菌活性。结果:以该法提取的蒲公英叶脂溶性成分约有36种。蒲公英叶脂溶性成分的总抗氧化活性及其对超氧阴离子自由基(O_2~-·)和羟基自由基(·OH)的清除率均随样品浓度的增加而增大,但总抗氧化活性、对O_2~-·和·OH的清除作用均低于维生素C。蒲公英叶脂溶性成分对金黄色葡萄球菌、大肠埃希菌、枯草芽孢杆菌和白色念珠菌具有较好的抑菌和杀菌作用,其最小抑菌浓度和最小杀菌浓度分别为0.5,1.72,2.46,3.58 g·L-1和0.84,1.88,3.62,4.74 g·L~(-1),尤其对金黄色葡萄球菌的抑菌和杀菌效果最好。结论:该研究表明蒲公英叶脂溶性成分的组成较为复杂,且有一定的抗氧化活性和良好的抑菌效果,可为蒲公英资源的开发利用提供参考和依据。
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| 关 键 词： | 蒲公英叶 气相色谱质谱联用仪 脂溶性成分 抗氧化活性 抑菌 |
| 收稿时间： | 2017/7/5 0:00:00 |
GC-MS Analysis and Activity Research of Liposoluble Constituents from Taraxacum mongolicum Leaves |
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| DUAN Hong-bo,HUAN Chen-chen,LIANG Yin-ku,XU Zhong-yang and REN Xiao-feng.GC-MS Analysis and Activity Research of Liposoluble Constituents from Taraxacum mongolicum Leaves[J].China Journal of Experimental Traditional Medical Formulae,2018,24(1):60-66. |
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| Authors: | DUAN Hong-bo HUAN Chen-chen LIANG Yin-ku XU Zhong-yang and REN Xiao-feng |
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| Institution: | Shaanxi Province Key Laboratory of Bio-Resources, Shaanxi University of Technology, Hanzhong 723000, China;School of Biological Science & Engineering, Shaanxi University of Technology, Hanzhong 723000, China,Shaanxi Province Key Laboratory of Bio-Resources, Shaanxi University of Technology, Hanzhong 723000, China;School of Biological Science & Engineering, Shaanxi University of Technology, Hanzhong 723000, China,Shaanxi Province Key Laboratory of Bio-Resources, Shaanxi University of Technology, Hanzhong 723000, China;School of Biological Science & Engineering, Shaanxi University of Technology, Hanzhong 723000, China,Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, China and Hanzhong TRG Biotechnology Co. Ltd., Hanzhong 723000, China |
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| Abstract: | Objective:To study the composition, antioxidant activity and antimicrobial activity of liposoluble constituents from Taraxacum mongolicum leaves. Method:Soxhlet extraction was used to extract the liposoluble constituents from dandelion leaves (LCDLs), and their compositions were studied by using GC-MS. The antioxidant activity was determined by phosphomolybdate complex method, pyrogallol autoxidation spectrophotometry and Fenton oxidation method, while the antimicrobial activity was determined by the slip method and microporous diffusion method. Result:There were about 36 kinds of LCDLs extracted by this method. With the increase of sample concentration, the total antioxidant activity of LCDLs and their clearance for the superoxide anion (O2-·) and hydroxyl radical (·OH) were all enhanced. However, their total antioxidant activity and scavenging effect on the O2-·and·OH were lower than those of Vit C(VC). LCDLs had good antibacterial and bactericidal effect against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Candida albicans, with MIC and MBC of 0.5, 1.72, 2.46, 3.58 g·L-1 and 0.84, 1.88, 3.62 and 4.74 g·L-1 respectively. Especially, it showed best antimicrobial and bactericidal effect against S. aureus. Conclusion:This study showed that the LCDLs were complex and had a certain antioxidant activity and good antimicrobial effect, providing reference and basis for the development and utilization of T. mongolicum resources. |
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| Keywords: | Taraxacum mongolicum leaves GC-MS liposoluble constituents antioxidant activity antimicrobial |
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