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乌头叶面微生物菌群变化特征及其与乌头霜霉病相关性
引用本文:李娜,胡亮,王婷,欧洪,王光志.乌头叶面微生物菌群变化特征及其与乌头霜霉病相关性[J].中国实验方剂学杂志,2017,23(5):42-46.
作者姓名:李娜  胡亮  王婷  欧洪  王光志
作者单位:成都中医药大学, 中药材标准化教育部重点实验室, 中药资源系统研究与 开发利用省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学, 中药材标准化教育部重点实验室, 中药资源系统研究与 开发利用省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学, 中药材标准化教育部重点实验室, 中药资源系统研究与 开发利用省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学, 中药材标准化教育部重点实验室, 中药资源系统研究与 开发利用省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学, 中药材标准化教育部重点实验室, 中药资源系统研究与 开发利用省部共建国家重点实验室培育基地, 成都 611137
基金项目:国家自然科学基金项目(30901962);四川省教育厅重点项目(15ZA0098)
摘    要:目的:以四川江油栽培乌头为研究对象,探索乌头叶面微生物菌群动态变化特征及其与乌头霜霉病的相关性。方法:收集不同病害等级的乌头霜霉病叶片,摇床震荡与超声结合的方法提取乌头叶面微生物,稀释平板计数法测定乌头叶面细菌,真菌数量。结果:乌头霜霉病发病情况与环境温度相关,患病乌头叶面细菌,真菌数量相较于健康乌头都有所增加,且叶面其他真菌数量随着病害程度的逐渐加重而呈现出较有规律的逐渐增加趋势。结论:乌头霜霉病在其生长期的3月末至5月末发病迅速,且日平均气温15~20℃比较适宜乌头霜霉病病原菌的生长繁殖。乌头叶面真菌数量的变化与乌头霜霉病病害程度呈正相关。

关 键 词:乌头  霜霉病  叶面  细菌  真菌
收稿时间:2016/7/25 0:00:00

Variation Characteristics of Phylloplane Microbies from Aconitum carmichaeli and Correlation with Downy Mildew
LI N,HU Liang,WANG Ting,OU Hong and WANG Guang-zhi.Variation Characteristics of Phylloplane Microbies from Aconitum carmichaeli and Correlation with Downy Mildew[J].China Journal of Experimental Traditional Medical Formulae,2017,23(5):42-46.
Authors:LI N  HU Liang  WANG Ting  OU Hong and WANG Guang-zhi
Institution:Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Co-founded by Sichuan Province and Ministry of Science and Technology, Ministry of Education, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China,Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Co-founded by Sichuan Province and Ministry of Science and Technology, Ministry of Education, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China,Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Co-founded by Sichuan Province and Ministry of Science and Technology, Ministry of Education, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China,Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Co-founded by Sichuan Province and Ministry of Science and Technology, Ministry of Education, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China and Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Co-founded by Sichuan Province and Ministry of Science and Technology, Ministry of Education, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
Abstract:
Keywords:Aconitum carmichaeli  downy mildew  phylloplane  bacteria  fungi
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