玉郎伞黄酮对心肌缺血再灌注损伤心肌组织ATP酶和凋亡蛋白的影响

目的 :研究玉郎伞黄酮(FYLS)对大鼠离体心肌缺血再灌注损伤(I/R)心肌组织Na⁺-K⁺-ATP酶、Ca²⁺-ATP酶和凋亡蛋白的影响。 方法 :离体大鼠心脏采用Langendorff法灌流,停灌30 min再灌30 min造成I/R模型。测定心肌组织Na⁺-K⁺-ATP酶、Ca²⁺-ATP酶活性。同时观察药物对缺血再灌注后心肌组织病理形态、凋亡相关蛋白Bcl-2,Bax表达的影响。 结果 :FYLS高剂量(8×10^-3g ·L^-1)可显著改善缺血-再灌注所致的心功能损伤,增加Na⁺-K⁺-ATP酶、Ca²⁺-ATP酶的活性;与模型组比较,FYLS高剂量组病理结果显示心肌组织受损程度明显减轻(P<0.05),且明显降低心肌Bax蛋白表达(P<0.01),对Bcl-2蛋白表达无明显影响,但能显著增加Bcl-2/Bax的比率(P<0.05)。 结论 :FYLS对I/R具有保护作用,其机制可能与减轻钙超载、下调Bax蛋白表达、增加Bcl-2/Bax的比率有关。

Effect of Flavonoid of Yulangsan on ATPase and Apoptosis-correlated Protein of Myocardial Ischemia-Reperfusion Injury

Objective: To study the effect and mechanism of flavonoid of Yulangsan (FYLS) on the activities of Na⁺-K⁺-ATPase and Ca²⁺-ATPase and the apoptosis-correlated proteins of myocardial ischemia-reperfusion injury (I/R) in isolated rat hearts. Method: Langendorff-perfusion Isolated rat hearts were prepared. I/R was induced by 30 minutes global ischemia followed by 30 minutes reperfusion. The activities of Na⁺-K⁺-ATPase and Ca²⁺-ATPase in myocardial tissues were determined. The pathological changes of myocardium and the expressions of apoptosis-correlated protein Bcl-2 and Bax were analyzed. Result: High dose of FYLS(8×10^-3g ·L^-1)could increase the activities of Na⁺-K⁺-ATPase and Ca²⁺-ATPase. Histopathological examination confirmed that the degree of myocardial injury in high dose of FYLS group was obviously lessened when compared with I/R group (P<0.05). FYLS could downregulate the expression of Bax (P<0.01) and significantly increase the ratio of Bcl-2/Bax (P<0.05). Conclusion: FYLS may offer myocardial protective effect against I/R, which may be related to its effects of reducing calcium overload, downregulating the expression of Bax protein, and increasing the Bcl-2/Bax ratio.