鱼腥草总生物碱的大孔吸附树脂纯化工艺

目的:优选鱼腥草总生物碱大孔吸附树脂的分离纯化工艺.方法:以盐酸小檗碱、胡椒内酰胺及马兜铃内酰胺B含量为指标,比较不同型号树脂和不同工艺条件对鱼腥草总生物碱的分离纯化能力.结果:HPD-100型大孔树脂对鱼腥草总生物碱有良好的吸附分离性能,最佳工艺条件为药液质量浓度20 g?L-1,pH 3,吸附流速0.5 mL? min -1,洗脱溶剂为氨水乙醇溶液(氨水浓度0.5 mol?L-1,乙醇体积分数80％),洗脱剂用量4 BV,洗脱速度3.0mL?min -1.结论:HPD-100型大孔树脂可用于鱼腥草总生物碱的提取纯化.

Purification Process of Total Alkaloids from Houttuynia cordata by Macroporous Resin

Objective: To optimize separation and purification technology of total alkaloids from Houttuynia cordata by macroporous resin. Method: With the content of berberine hydrochloride, pepper lactam and aristololactam B as indexes,separation and purification capacity of total alkaloids from H. cordata was compared with different types of macroporous resin and different technology conditions. Result: HPD-100 macroporous resin had the best separation and purification capacity for total alkaloids in following technology conditions: sample concentration was 20 g·L^-1 with pH 3, flow rate was 0.5 mL·min^-1,eluting reagent was ethanol solution of ammonia(ammonia concentration 0.5 mol·L^-1,the volume fraction of ethanol 80%) with the amount of 4 BV,eluting velocity was 3.0 mL·min^-1. Conclution: HPD-100 macroporous resin could be applied to purify alkaloids from H. cordata.