广西藤茶双氢杨梅树皮素对小鼠肝细胞凋亡的影响

目的:探讨藤茶双氢杨梅树皮素(Vine tea dihydromyricetin,VTD)对撤除苯巴比妥钠(sodium Phenobarbital,SP)诱导的小鼠肝细胞凋亡的影响,揭示其抗肝损伤作用的机制。方法:采用撤除SP诱导肝细胞凋亡的小鼠模型,予以高、低剂量VTD(200,50 mg·kg-1)ig,并设置正常对照、SP不撤对照及SP撤除模型组。以HE染色镜检观察肝组织病理形态变化,以流式细胞术、细胞原位TUNEL法检测肝细胞凋亡率,评估VTD对肝细胞凋亡的影响。结果:HE染色镜检可见正常对照组与SP不撤对照组肝组织形态大致正常,SP撤除模型组与VTD高、低剂量组均见不同程度肝细胞凋亡。流式细胞术分析、细胞原位TUNEL法检测结果显示,VTD低剂量组肝细胞凋亡率分别为17.7%,2.9%,均显著低于SP撤除模型组(分别为27.4%,4.5%),差异具有统计学意义(P0.01)。VTD高剂量组肝细胞凋亡率与SP撤除模型组比较其差异无统计学意义。结论:50mg·kg-1·d-1的VTD ig可显著抑制SP撤除诱导的小鼠肝细胞凋亡,提示抑制肝细胞凋亡可能为VTD肝保护作用的机制之一。

Effects of Dihydromyricetin from Guangxi Vine Tea on Hepatocelluar Apoptosis in Mice

Objective: To study the inhibitory effect of dihydromyricetin from Ampelopsis grossedentata (Hand-Mazz) W.T. Wang (Vine tea dihydromyricetin,VTD) on hepatocyte apoptosis induced by the cessation of phenobarbital sodium (SP) injection treatment in mice,and to explore its mechanism of hepatoprotective effect. Method: The mouse model with hepatocellular apoptosis induced by removing SP was established.The mice were randomly divided into blanks,negative controls,positive controls and test groups.Test groups of mice were given intragastric gavage of 200, 50 mg ·kg^-1 VTD.Hepatic apoptosis was detected by flow cytometry,terminal deoxynucleotidyl transferase dUTP nick-end(TUNEL),and pathological changes in the liver tissue were observed after HE staining. Result: The histopathological analysis of the liver showed normal liver morphology in blanks and negative controls,and varying degrees of liver cell apoptosis in positive controls and low- and high-dose VTD groups.The outcome from flow cytometry and TUNEL showed hepatocelluar apoptosis rate in low dose of VTD group were 17.7%, 2.9% respectively.All of these values were lower than those in positive controls (P<0.01). Conclusion: The mouse hepatocellular apoptosis induced by removing SP can be markedly inhibited by treating with VTD in 50 mg ·kg^-1,and it might be one of the hepatoprotective mechanisms for VTD to inhibit the hepatocelluar apoptosis.