运用熵权模糊综合评判模型评价几种促透剂的作用效果

目的:考察熵权模糊综合评判模型对促透剂促透效果评价的可行性,为促透剂评价提供可靠的方法。方法:以双氯芬酸钠为模型药物,氮酮、陈皮挥发油、桉叶油、薄荷醇和冰片为促透剂,以离体SD大鼠皮肤为透皮屏障,在改良的Franz扩散池上进行透皮试验。采用HPLC测定接收液中双氯芬酸钠含量,流动相甲醇-水-冰乙酸(80∶20∶0.5),检测波长276 nm,计算渗透系数、稳态流量、滞后时间、增渗倍数,运用熵权模糊综合评判模型对各促透剂的作用进行评价。结果:各组促透剂对双氯芬酸钠均有不同效果的促透作用,其中氮酮、薄荷醇和冰片表现为优,最大隶属度分别为0.761 1,0.582 1和0.495 4;陈皮挥发油和桉叶油表现为良,最大隶属度分别为0.454 2和0.480 9。结论:熵权模糊综合评判模型可对中药促透剂的促透效果进行客观、清晰的评价。

Investigation of Promoting Effects of Some Penetration Enhancers by Entropy Weight Fuzzy Comprehensive Evaluation Model

Objective: To investigate whether entropy weight fuzzy comprehensive evaluation method is feasible for study on promoting effects of some kinds of penetration enhancers and to provide a reliable evaluation method for penetration enhancers. Method: Diclofenac sodium was used as model drug, azone, volatile oil from Citri Reticulatae Pericarpium, eucalyptus oil, menthol and borneol were tested as transdermal enhancers, taking isolated skin of SD rats as transdermal barrier, transdermal experiments were carried by a modified Franz diffusion cell.The content of diclofenac sodium was measured by HPLC with mobile phase of methanol-water-acetic acid (80: 20: 0.5) and detection wavelength at 276 nm.Permeability coefficient, lag time, steady flow and enhancement ratio were calculated.Effects of penetration enhancers were evaluated by entropy weight fuzzy comprehensive evaluation method. Result: Penetration enhancers had penetration enhancing effect on diclofenac sodium with different degrees.Azone, menthol and borneol had the best significant promoting effects whose maximum memberships were 0.761 1, 0.582 1 and 0.495 4;volatile oil from Citri Reticulatae Pericarpium and eucalyptus oil had certain promoting effects whose maximum memberships were 0.454 2 and 0.480 9. Conclusion: Entropy weight fuzzy comprehensive evaluation method is feasible for evaluating promoting effects of penetration enhancers.