仙慈丹有效部位对人肺癌细胞A549增殖的抑制作用

目的:探讨仙慈丹(XCD)有效部位对肺癌细胞A549增殖抑制作用及其作用机制.方法:XCD有效部位为XCD水提滤液通过AB-8型大孔树脂柱收集80％乙醇洗脱液所得,得率为0.90％.体外培养A549细胞至对数期,分别以质量浓度为5,10,15,25,50,100,200 mg·L-1的仙慈丹有效部位作用,应用MTT法测定其对A549细胞的抑制率,进而求出半数抑制浓度(IC50).运用AnnexinV-FITC/PI双染流式细胞术及PI单染流式细胞术测定XCD有效部位对A549细胞凋亡及周期的影响.结果:通过MTT实验,经过统计计算得出XCD有效部位在对A549作用24,48,72 h后的IC50分别为136.550,56.671,55.322 mg·L-1；通过流式细胞仪测定XCD作用下A549在24,48,72 h的凋亡率分别为(17.98 ±0.11)％,(23.34 ±0.23)％,(29.54±0.78)％；XCD作用A549细胞24 h后,细胞周期被阻滞在S期,在48 h后细胞周期被阻滞在G0/G1期.结论:XCD有效部位对A549细胞增殖抑制作用明显,通过诱导肿瘤细胞凋亡,同时调节细胞周期抑制A549细胞生长.

Effect of Xiancidan on Apoptosis and Cycle of non-small Cell Lung Cancer A549 in vitro

Objective: To investigate the effects of Xiancidan(XCD) effective parts on inhibition of lung cancer A549 in vitro,and explore its mechanism. Method: The A549 cells in vitro were treated with the XCD effective parts at different concentrations, the concentrations of the XCD effective parts were 5,10,15,25,50,100,200 mg ·L^-1 respectively. The inhibitory rate of the A549 cell proliferation was measured by MTF assay, the IC₅₀ (the 50% inhibitory concentration)was determined. The effects of XCD effective parts on A549 cell cycle and cell apoptosis were determined by Annexin V-FITC/PI double staining assay. Result: MTT assay was used to calculate XCD effective parts in A549 role for 24, 48, 72 h. the 50% inhibitory concentration(IC₅₀) of the 3 manners above were 136.550,56.671,55.322 mg·L^-1 respectively. Flow cytometry was used to measured the XCD effective parts in A549 role for 24, 48, 72 h. The rates of apoptosis of the above 3 manners were(17.98±0.11)%,(23.34±0.23)%,(29.54±0.78)% respectively. After treated by XCD for 24 h, A549 cells were arrested in S phase, and for 48 h in G₀/G₁ phase. Conclusion: The XCD effective parts can inhibit the growth of A549 cells remarkably by inducing apoptosis and blocking cell cycle.