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| 山茱萸多糖诱导宫颈癌细胞凋亡及Bax蛋白表达的变化 | |
| 引用本文： | 王恩军,靳祎,王哲,杨欢欢,周艳芬,刘斌.山茱萸多糖诱导宫颈癌细胞凋亡及Bax蛋白表达的变化[J].中国实验方剂学杂志,2012,18(10):260-262. |
| 作者姓名： | 王恩军 靳祎 王哲 杨欢欢 周艳芬 刘斌 |
| 作者单位： | 1. 河北大学,河北保定,071000 2. 保定市第二医院,河北保定,071051 3. 保定市急救中心医院,河北保定,071000 |
| 基金项目： | 河北省人口和计划生育委员会科研计划项目(2009-B18),保定市科技局科学技术研究与发展指导计划项目(09ZF011) |
| 摘 要： | 目的:探讨山茱萸多糖诱导人宫颈癌Hela细胞的凋亡作用及Bax蛋白表达的变化.方法:用12.5,25,50 g·L-1的山茱萸多糖作用Hela细胞24 h.倒置显微镜下观察细胞凋亡的形态,免疫组化SP法检测凋亡相关蛋白Bax表达的变化.结果:Hela细胞经3个不同浓度的山茱萸多糖作用后,出现典型的细胞凋亡的形态学变化,其程度与浓度相关；而且Bax蛋白的表达量随浓度升高而增加,免疫组化评分(IHS)分别为0.18,0.44,1.35.结论:山茱萸多糖能够促进Bax的表达,诱导Hela细胞凋亡. |
| 关 键 词： | 山茱萸多糖 宫颈癌 Hela细胞 Bax 凋亡 免疫组化 |
| 收稿时间： | 2011/12/30 0:00:00 |
The Expression of Bax Proteinum of Fructus Corni Polysaccharides in Human Cervical Cancer Cells |
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| WANG En-jun,JIN Yi,WANG Zhe,YANG Huan-huan,ZHOU Yan-fen and LIU Bin.The Expression of Bax Proteinum of Fructus Corni Polysaccharides in Human Cervical Cancer Cells[J].China Journal of Experimental Traditional Medical Formulae,2012,18(10):260-262. | |
| Authors: | WANG En-jun JIN Yi WANG Zhe YANG Huan-huan ZHOU Yan-fen and LIU Bin |
| Institution: | Hebei University, Baoding 071000, China;Hebei University, Baoding 071000, China;The Number Two Hospital, Baoding 071051, China;Hebei University, Baoding 071000, China;Hebei University, Baoding 071000, China;Baoding Medical Emergency Center, Baoding 071000, China |
| Abstract: | Objective: To expLore the apoptosis effect of fructus corni polysaccharides on expression of Bax in Hela cells. Method: Hela cells were treated with various concentration of fructus corni polysaccharides for 24 h, the change of cells' apoptosis shape was observed by upside-down microscope,and expression of Bax was detected by immunohistochemistry streptaridin peroxidase. Result: Morphological observation appeared representative cell apoptosis and Bax expression up after fructus corni polysaccharides on Hela cells. Its degree was correlated with concentration. Conclusion: Fructus corni polysaccharides can promote Bax expression and induce cell apoptosis. |
| Keywords: | Fructus corni polysaccharides cervical cancer Hela cells Bax apoptosis immunohistochemistry |
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