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补中益气汤对甲状腺功能减退大鼠心肌细胞凋亡及 Fas, FasL和Caspase-3蛋白表达的影响
引用本文:高天舒,韩晓晴,尹慧丝.补中益气汤对甲状腺功能减退大鼠心肌细胞凋亡及 Fas, FasL和Caspase-3蛋白表达的影响[J].中国实验方剂学杂志,2012,18(10):236-240.
作者姓名:高天舒  韩晓晴  尹慧丝
作者单位:1. 辽宁中医药大学附属医院内分泌科,沈阳,110032
2. 辽宁中医药大学,沈阳,110032
基金项目:辽宁省教育厅项目(2009A492);辽宁省教育部博士点基金项目(20092133110004);辽宁省教育厅优秀人才计划项目(LR201026)
摘    要:目的:研究补中益气汤对甲状腺功能减退(甲减)大鼠心肌细胞凋亡及Fas,FasL和Caspase-3蛋白表达的影响.方法:采用Kolaja法制备大鼠甲状腺功能减退模型.健康Wistar大鼠120只随机分成正常对照组、模型组、补中益气汤组(18.9 g·kg-1)、左旋甲状腺素钠L-T4组(9.45μg·kg-1),各组灌胃给药56 d.光镜和电镜下观察心肌形态学和超微结构变化,TUNEL法检测心肌凋亡细胞,免疫组化检测凋亡相关基因蛋白Fas,FasL,Caspase-3的表达,ELISA法测定血清促甲状腺激素(TSH),放射免疫分析法测定血清总三碘甲状腺原氨酸(Tr3)及总甲状腺素(TT4).结果:与正常组相比,模型组心肌细胞凋亡指数及Fas,FasL,Caspase-3的表达均达高峰,心肌细胞结构损伤严重,经治疗后补中益气汤组(12.10±0.73)和L-T4组(29.26±2.10)的心肌细胞凋亡指数降低,Fas,FasL及Caspase-3的阳性表达均下降,且补中益气汤组的疗效优于L-T4组(P<0.05).结论:甲减引起心肌细胞凋亡增多,Fas,FasL,Caspase-3表达升高,而补中益气汤可明显降低心肌细胞凋亡指数,下调Fas,FasL及Caspase-3的表达,说明补中益气汤对甲减的心肌损害有干预保护作用.

关 键 词:补中益气汤  甲状腺功能减退  细胞凋亡  Fas  FasL  Caspase-3
收稿时间:2011/12/26 0:00:00

Influence of Buzhong Yiqi Tang on Myocardial Apoptosis and Expression of Proteins Fas, FasL and Caspase-3 in Rats of Hypothyroidism
GAO Tian-shu,HAN Xiao-qing and YIN Hui-si.Influence of Buzhong Yiqi Tang on Myocardial Apoptosis and Expression of Proteins Fas, FasL and Caspase-3 in Rats of Hypothyroidism[J].China Journal of Experimental Traditional Medical Formulae,2012,18(10):236-240.
Authors:GAO Tian-shu  HAN Xiao-qing and YIN Hui-si
Institution:Endocrinology Department of Affiliated lespital of LiaoNing University of Traditional Chinese Medicine, Shenyang 110032, China;LiaoNing University of Traditional CHinese Medicine, Shenyang 110032, China;LiaoNing University of Traditional CHinese Medicine, Shenyang 110032, China
Abstract:Objective: To study the effect of Buzhong Yiqi Tang(tonifying middle-Jiao and Qi treatment) on myocardial apoptosis and expression of proteins Fas, FasL and Caspase-3 in hypothyroid rats. Method: The rat of hypothyroidism models were prepared by Kolaja method.Healthy wistar rats were randomly divided into normal control group,model group, Buzhong Yiqi Tang group, Letrox(L-T4)group. The morphological and ultrastructural changes were observed under optical microscope and electronic microscope respectively. Apoptotic cells were measured by TdT-mediated dUTP nick end labeling (TUNEL) method.The expression of apoptosis-related proteins Fas, FasL and Caspase-3 were detected with immunohistochemical methods. TSH was measured by ELISA, Totel 3 Triodothy ronine(TT3)and Totel 4 Triodothy ronine (TT4) were measured by RIA;detect the iodine in urine. Result: Compared with control group,the apoptosis index and the expression of Fas, FasL and Caspase-3 reached the highest level in the model group,myocardial cell injured severely. After treatment the apoptosis index of Buzhong Yiqi Tang group(12.10±0.73) and L-T4 group(29.26±2.10) decreased,the expression of Fas, FasL and Caspase-3 also reduced,and the effectiveness of Buzhong Yiqi Tang group was better(P<0.05). Conclusion: Hypothyroidism causes increase of myocardial cell apoptosis,heightened expression of Fas, FasL and Caspase-3, while Buzhong Yiqi Tang might decrease the myocardial apoptosis and lower the expression of protein Fas, FasL and Caspase-3 significantly,it indicated that Buzhong Yiqi Tang has protective action on myocardial injury of hypothyroidism.
Keywords:Buzhong Yiqi Tang  hypothyroidism  cell apoptosis
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