| 红花多糖的提取与含量测定 |
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| 引用本文： | 张晓莉,李玉婷,王亚贤,石学魁,李福娟,桂金秋.红花多糖的提取与含量测定[J].中国实验方剂学杂志,2010,16(7):19-21. |
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| 作者姓名： | 张晓莉 李玉婷 王亚贤 石学魁 李福娟 桂金秋 |
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| 作者单位： | 1. 牡丹江医学院病原生物学教研室,黑龙江,牡丹江,157011
2. 黑龙江中医药大学微生物与免疫学教研室,哈尔滨,150040 |
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| 摘 要： | 目的:研究红花多糖的提取及含量测定,为开展红花多糖的抗肿瘤作用机制提供理想药物。方法:采用水提醇沉法从红花药材中提取并分离出红花粗多糖,用Sevage法除去蛋白质,过氧化氢脱色;采用苯酚-硫酸显色,用分光光度法测定多糖含量。结果:红花多糖的浓度与吸光度在5～50μg·mL-1呈良好的线性关系,回归方程:Y=0.01749X-0.03165,r=0.9994。多糖质量分数为96.048%。结论:含量测定方法操作简便,灵敏度高,稳定,重现性好。该方法可为开展红花多糖的抗肿瘤作用研究提供理想的实验药物。
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| 关 键 词： | 红花多糖 分光光度法 提取 纯化 含量测定 |
| 收稿时间： | 2010/1/22 0:00:00 |
Extraction and Assaying of Safflower Polysaccharide |
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| ZHANG Xiao-li,LI Yu-ting,WANG Ya-xian,SHI Xue-kui,LI Fu-juan and GUI Jin-qiu.Extraction and Assaying of Safflower Polysaccharide[J].China Journal of Experimental Traditional Medical Formulae,2010,16(7):19-21. |
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| Authors: | ZHANG Xiao-li LI Yu-ting WANG Ya-xian SHI Xue-kui LI Fu-juan and GUI Jin-qiu |
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| Institution: | Teaching and Research Section of Pathogenic Biology,Mudanjiang Medical College, Mudanjiang 157011, China;Teaching and Research Section of Pathogenic Biology,Mudanjiang Medical College, Mudanjiang 157011, China;Department of Microbiology and Immunology, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China;Teaching and Research Section of Pathogenic Biology,Mudanjiang Medical College, Mudanjiang 157011, China;Teaching and Research Section of Pathogenic Biology,Mudanjiang Medical College, Mudanjiang 157011, China;Teaching and Research Section of Pathogenic Biology,Mudanjiang Medical College, Mudanjiang 157011, China |
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| Abstract: | |
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| Keywords: | safflower polysaccharide(SPS) spectrophotography extraction purification assaying |
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