右归丸补肾填精的药理作用

目的:以下丘脑-垂体-性腺轴为基础,探究右归丸对肾阳虚大鼠的药理作用,探讨其补肾填精的作用机制。方法:SD大鼠60只,采用氢化可的松建立肾阳虚模型大鼠,随机分为6组,分别为正常组、模型组、甲基睾酮(0.5 g·kg-1)组、右归丸低、中、高剂量组(0.5,1.0,2.5 g·kg-1),每组10只。造模后连续ig给药30 d,分别于给药第15,30天进行强迫负重游泳实验;第30天,眼球取血,放射免疫分析法检测大鼠血清睾酮(testosterone,T)、雌二醇(estradiol,E2)的含量;采用RT-PCR法检测各组大鼠下丘脑、垂体和靶腺(睾丸)钙调蛋白(Ca M)mRNA的表达。结果:与正常组比,模型组大鼠的负重游泳时间减少(P0.05),明显升高血清中E2的激素水平及降低T的激素水平(P0.05),明显升高下丘脑、垂体、睾丸中Ca M mRNA的表达(P0.05);与模型组比,甲基睾酮组、右归丸低、中、高剂量组可明显提高肾阳虚模型大鼠的负重游泳时长(P0.05),给药20 d后其体能趋于正常;给药20 d后甲基睾酮组、右归丸低、中、高剂量组可明显降低血清中E2的激素水平及升高T的激素水平(P0.05),明显降低下丘脑、垂体、睾丸中Ca M mRNA的表达(P0.05)。结论:右归丸可通过调节机体下丘脑-垂体-性腺轴中钙调蛋白基因表达,改善阳虚大鼠激素水平,逆转肾阳虚状态,具有补肾填精的作用。

Effect Study of Yougui Wan on Tonifying Kidney

Objective: To explore the pharmacological effect and possible meachanism of Yougui Wan on Shen yang deficiency based on hypothalamus-pituitary-gonad axis. Method: The Shen yang deficiency model rats were induced by hydrocortisone. The 60 rats were randomly divided into six groups:the control group, the model group, the methyltestosterone group (0.5 g·kg^-1), low-, middle-and high-dose Yougui Wan groups (0.5, 1.0, 2.5 g·kg^-1). Changes of forced swimming time were tested at 15 and 30 days. Serum levels of testosterone (T) and estradiol (E₂) were analyzed by immunobiochemistry, and calmodulin (CaM) mRNA in hypothalamus-pituitary-gonad axis of rats in all groups were determined via quantitative RT-PCR technique after 30 days. Result: The forced swimming time decreased, blood concentration of E₂ increased, blood concentration of T decreased, and CaM mRNA levels in rat hypothalamus, pituitary and testicle increased in the model group as compared with the normal group (P<0.05). All above indexes had good improvement in methyltestosterone group and all doses Yougui Wan groups as compared with the model group (P<0.05). Conclusion: Yougui Wan have therapeutical effect on down-regulating the CaM mRNA levels of testical and hypothalamus, regulating the levels of E₂ and T, and improving the symptoms of Shen yang deficiency.