| 苯酚-硫酸法测定乌梅多糖的含量 |
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| 引用本文： | 张璐,翁立冬,刘莉,刘强.苯酚-硫酸法测定乌梅多糖的含量[J].中国实验方剂学杂志,2011,17(6):107-109. |
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| 作者姓名： | 张璐 翁立冬 刘莉 刘强 |
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| 作者单位： | 南方医科大学中医药学院,广州,510515 |
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| 摘 要： | 目的:建立乌梅药材中多糖的含量测定方法。方法:采用苯酚-硫酸显色法测定乌梅药材中多糖的含量。结果:样品经苯酚-硫酸显色后于488 nm处测定吸收度,在此波长处溶液的吸收度与葡萄糖含量呈良好的线性关系;线性范围0.02～0.2 g·L-1(r=0.999 8),加样回收率100.38%,RSD 0.892%;乌梅中多糖含量2.51%。结论:苯酚-硫酸显色法操作简便,结果准确,为乌梅多糖的进一步研究奠定良好的基础。
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| 关 键 词： | 乌梅 多糖 苯酚-硫酸法 |
| 收稿时间： | 9/2/2010 12:00:00 AM |
Determination of Content of Polysaccharides in Prunus Mume by Phenol-sulfuric Method |
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| ZHANG Lu,WENG Li-dong,LIU li and LIU Qiang.Determination of Content of Polysaccharides in Prunus Mume by Phenol-sulfuric Method[J].China Journal of Experimental Traditional Medical Formulae,2011,17(6):107-109. |
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| Authors: | ZHANG Lu WENG Li-dong LIU li and LIU Qiang |
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| Institution: | College of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China;College of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China;College of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China;College of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | Objective: To establish an analysis method for the determination of the content of polysaccharides from Prunus mume. Method: Using Phenol-sulfuric Method to determination of the content of polysaccharides for P.mume. Result: The absorbance was determined at 488 nm. The linear curves was fine between the concentration rang of 0.02-0.2 g ·L-1 (r=0.999 8), the average recovery was 100.38%, the RSD was 0.892%.The content of polysaccharide in P.mume was 2.51%. Conclusion: Phenol-sulfuric method is a simple operation process with accurate result, laying a foundation for further study on polysaccharides in P.mume. |
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| Keywords: | Prunus mume polysaccharides phenol-sulfuric method |
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