白芍提取物对嗅球损毁抑郁模型大鼠 行为学及下丘脑-垂体-肾上腺轴的影响

目的:研究白芍提取物对嗅球损毁抑郁模型大鼠行为学及下丘脑-垂体-肾上腺(HPA)轴的影响。方法:将嗅球损毁大鼠随机分为对照组、模型组、阳性药氟西汀2.5 mg.kg-1组以及白芍提取物70,35,17.5 mg.kg-1组,采用敞箱法、跳台法观察嗅球损毁大鼠的行为变化,同时用放免法分析白芍提取物对嗅球损毁大鼠下丘脑促肾上腺皮质激素释放素(CRH)、垂体促肾上腺皮质激素(ACTH)和血清皮质酮(CORT)含量的影响。结果:大鼠嗅球损毁后敞箱行为出现明显变化,水平运动和垂直运动显著增加,白芍提取物中、高剂量组可显著降低大鼠水平运动和垂直运动的得分;跳台试验中,造模后大鼠训练期和测试期的错误次数显著增加,白芍提取物中、高剂量组能显著降低嗅球损毁大鼠训练期和测试期的触电次数;嗅球损毁大鼠下丘脑CRH,垂体ACTH,血清CORT含量明显增加,白芍提取物中、高剂量组能显著降低嗅球损毁大鼠CRH,ACTH,CORT的含量。结论:白芍提取物能明显改善嗅球损毁大鼠的行为活动,并且对嗅球损毁大鼠亢进的HPA轴功能的调整作用是白芍提取物治疗抑郁症的机制之一。

Influences of Extract of Peony Radix Alba on Behavior and Hypothalamic-pituitary-adrenocortical Axis in Depressive Rats Model with Damaged Olfactory Bulb

Objective: To investigate the influences of extract from Peony Radix Alba on the behavioral and hypothalamic-pituitary-adrenocortical (HPA) axis changes in rats with damaged olfactory bulb (DOB). Method: The tests of open-field and step-down passive avoidance were used to observe the behaviors of rats. Radioimmunoassay (RIA) was used to analyze the level of corticotropin releasing hormone (CRH) in hypothalamus, adrenocorticotropic hormone (ACTH) in pituitary gland and cortisol (CORT) in serum of rats with DOB. Result: The rats had a characteristic hyperactivity in the test of "open-field" and learning deficits in step-down passive avoidance (P<0.05), and their levels of CRH, ACTH and CORT increased significantly (P<0.05). The extract of Peony Radix Alba at the dose of 70,35 mg ·kg^-1 corrected the behavioral changes (P<0.05) and decreased the levels of CRH, ACTH and CORT (P<0.05). Conclusion: The extract of Peony Radix Alba can correct behavioral changes in rats with DOB, and its regulating effect on HPA axis is one of the mechanisms for treating depression.