首页 | 本学科首页   官方微博 | 高级检索  
检索        

夏龙方对人胃癌SGC-7901细胞黏附和侵袭的影响
引用本文:刘威,沈克平,胡兵,潘传芳.夏龙方对人胃癌SGC-7901细胞黏附和侵袭的影响[J].中国实验方剂学杂志,2011,17(2):192-195.
作者姓名:刘威  沈克平  胡兵  潘传芳
作者单位:上海中医药大学附属龙华医院肿瘤五科,上海,200032
基金项目:国家自然科学基金,上海市浦东新区中医领军型人才项目,上海市级医院临床科研资源共享平台
摘    要:目的:观察夏龙方对胃癌细胞SGC-7901黏附、侵袭、移动等转移生物学行为的影响。方法:体外培养SGC-7901细胞,分为夏龙方50,100,200 mg·L-1(低、中、高剂量)组和对照组,采用Cytoselect 48-Well Cell Adhesion Assay检测胃癌细胞与基质黏附能力;利用Transwell侵袭转移模型评价夏龙方对胃癌细胞侵袭能力的影响、划痕实验观察夏龙方对SGC-7901细胞移动能力的影响。结果:终浓度100,200 mg·L-1夏龙方能显著降低SGC-7901细胞黏附能力,50~200 mg·L-1可抑制SGC-7901细胞侵袭,终浓度50 mg·L-1夏龙方可显著抑制SGC-7901细胞迁移。结论:夏龙方可降低SGC-7901细胞的黏附能力,侵袭能力,抑制细胞迁移。

关 键 词:夏龙方  中药复方  胃癌  黏附  侵袭  移动
收稿时间:2010/9/23 0:00:00

Eeffects of Xialongfang on Adhesion and Migration in SGC-7901 Gastric Carcinoma Cells
LIU Wei,SHEN Ke-ping,HU Bing and PAN Chuan-fang.Eeffects of Xialongfang on Adhesion and Migration in SGC-7901 Gastric Carcinoma Cells[J].China Journal of Experimental Traditional Medical Formulae,2011,17(2):192-195.
Authors:LIU Wei  SHEN Ke-ping  HU Bing and PAN Chuan-fang
Institution:5th Department of Oncology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 20032,China;5th Department of Oncology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 20032,China;5th Department of Oncology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 20032,China;5th Department of Oncology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 20032,China
Abstract:Objective: To investigate the influence of Xialongfang on adhesion,invasion and migration in SGC-7901 gastric carcinoma cells. Method: Human gastric carcinoma SGC-7901 cells were cultured in vitro ,and treated with different concentration Xialongfang(0-200 mg ·L-1 ).Cell adhesion was detected with cytoselect 48-well cell adhesion assay.Cell invasion was analyzed with Transwell invasion assay.Cell migration was detected with scratch wound healing assay. Result: 50-200 mg ·L-1 Xialongfang significantly inhibited the invasive capacities of SGC-7901 cells and inhibited cell adhesion at 100-200 mg ·L-1 .Wound healing assays showed a significant reduction in the migrated cells after treated for 24 h or 48 h at 50 mg ·L-1 Xialongfang. Conclusion: Xialongfang significantly decreased the adhesion and invasive capacities of SGC-7901 cell lines and inhibited cell migration.
Keywords:Xialongfang  Chinese herb formula  gastric carcinoma  adhesion  invasion  migration
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中国实验方剂学杂志》浏览原始摘要信息
点击此处可从《中国实验方剂学杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号