枇杷叶总黄酮大孔树脂纯化工艺优化

目的:优选大孔树脂吸附法富集枇杷叶总黄酮的工艺条件.方法:以芦丁为对照品,硝酸铝显色法检测枇杷叶总黄酮的含量.以静态吸附容量、静态解吸率为考察指标,比较6种大孔树脂对枇杷叶总黄酮的吸附和解吸效果,筛选最佳大孔树脂型号,并对其吸附和解吸条件进行探讨.结果:HPD100型大孔树脂最适合于枇杷叶总黄酮的纯化,其纯化工艺为上样液质量浓度3 g·L-1,上样速率2 BV·h-1,上样液体积2.5 BV,2 BV去离子水冲洗,5 BV 70％乙醇以1 BV·h-1流速洗脱,收集洗脱液.在此工艺条件下,总黄酮得率78.7％,总黄酮纯度47.3％.结论:HPD100型大孔树脂对枇杷叶总黄酮具有良好的纯化效果,优选工艺合理、稳定可行.

Optimization of Purification Process for Total Flavonoids from Leaves of Eriobotrya japonica by Macroporous Resin

Objective: To optimize enrichment technology conditions of total flavonoids from leaves of Eriobotrya japonica by macroporous adsorption resin. Method: With rutin as reference substance,the content of total flavonoids was determined by aluminium nitrate chromogenic method.With static adsorption capacity and static desorption rate as indexes,optimum type of macroporous resin was selected by comparing adsorption and desorption effect of 6 kinds macroporous resin for total flavonoids from leaves of E. japonica, and to investigate its absorption and desorption conditions. Result: HPD 100 macroporous resin was the most suitable for purification of total flavonoids from E. japonica.Optimum purification conditions were:the concentration of sampling liquid of 3 g·L^-1, sampling velocity 2 BV·h^-1, sample liquid volume 2.5 BV,eluted by 2 BV deionized water and 5 BV 70% ethanol at the speed of 1 BV·h^-1,eluate was collected.Under these process conditions,yield of total flavonoids was 78.7%,purity of total flavonoids was 47.3%. Conclusion: HPD 100 macroporous resin had a good purification effect of total flavonoids from leaves of E. japonica,optimized purification process was reasonable,stable and feasible.