| 苗药黑骨藤中总皂苷含量的测定 |
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| 引用本文： | 申海艳,龚小见,李文敏,赵杨,赵超,周欣.苗药黑骨藤中总皂苷含量的测定[J].中国实验方剂学杂志,2012,18(11):94-97. |
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| 作者姓名： | 申海艳 龚小见 李文敏 赵杨 赵超 周欣 |
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| 作者单位： | 贵州师范大学天然药物质量控制研究中心,贵阳550001;贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室,贵阳550001 |
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| 基金项目： | 贵州省中药现代化科技产业研究开发专项项目(黔科合社字[2009]5012号);贵州省科技创新人才团队建设项目(黔科合人才团队[2011]4008);贵阳市科学技术计划项目([2010]筑科 计 合同字第3-重-1号);贵阳市科技计划项目([2009]筑科农合同字第8-003号);贵州省中药材现代产业技术体系建设专项(GZCYTX-02);贵州省平台建设项目(黔科ZY[2011]3013号);贵阳市大学生科技创业计划项目([2011]08号) |
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| 摘 要： | 目的:建立苗药黑骨藤中总皂苷含量测定的方法,并对不同来源药材中总皂苷的含量进行测定.方法:以熊果酸为对照,用5％的香草醛-冰醋酸及高氯酸进行显色,在550 nm波长下运用紫外分光光度法对总皂苷含量进行测定.结累:熊果酸在9～55 mg·L-1线性关系良好,回归方程Y=0.011 9X +0.097 2(r =0.999 2),方法的平均回收率为98.74％,RSD1.2％；不同来源黑骨藤中总皂苷的含量存在差异.结论:该方法快速、简便,精密度和稳定性良好,可用于黑骨藤中总皂苷含量的测定.
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| 关 键 词： | 黑骨藤 紫外分光光度法 总皂苷 熊果酸 |
| 收稿时间： | 2011/11/3 0:00:00 |
Determination of Total Saponins in Periploca forrestii |
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| SHEN Hai-yan,GONG Xiao-jian,LI Wen-min,ZHAO Yang,ZHAO Chao and ZHOU Xin.Determination of Total Saponins in Periploca forrestii[J].China Journal of Experimental Traditional Medical Formulae,2012,18(11):94-97. |
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| Authors: | SHEN Hai-yan GONG Xiao-jian LI Wen-min ZHAO Yang ZHAO Chao and ZHOU Xin |
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| Institution: | The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China;Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001, China;The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China;Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001, China;The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China;Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001, China;The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China;Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001, China;The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China;Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001, China;The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China;Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001, China |
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| Abstract: | Objective: The quantitative method was established of total saponins in Periploca forrestii and the content of the general saponins of Periploca forrestii from different places was determined. Method: Ursolic acid was selected as control article.Coloration was used with 5% vanillin and perchloric acid.The content of total saponins in P. forrestii was determined at 550 nm by UV. Result: The calibration curve was linear over the range of 9-55 mg·L-1 with the correlation of 0.999 2,and the linear regression equation was A=0.011 9 C+0.097 2.The average recovery of ursolic acid was 97.16% with RSD of 1.3%(n=6). The contents of the total saponins in P. forrestii from various habitats was quite different. Conclusion: The method is accurate,simple,sensitive and reliable. So it can be used to determine the content of total saponins in P. forrestii. |
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| Keywords: | Periploca forrestii UV total saponins ursolic acid |
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