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| 一测多评法与外标法测定新清宁片中大黄蒽醌类成分含量 | |
| 引用本文： | 张锴镔,冯伟红,王智民,张启伟,李霞,杨菲,李东影,吉丽娜.一测多评法与外标法测定新清宁片中大黄蒽醌类成分含量[J].中国实验方剂学杂志,2012,18(11):61-65. |
| 作者姓名： | 张锴镔 冯伟红 王智民 张启伟 李霞 杨菲 李东影 吉丽娜 |
| 作者单位： | 1. 中国中医科学院中药研究所,北京 100700;中药质量控制技术国家工程实验室,北京 100700 2. 中国中医科学院实验药厂,北京,100700 3. 中国中医科学院中药研究所,北京 100700;中药质量控制技术国家工程实验室,北京 100700;天津中医药大学,天津300193 |
| 基金项目： | 2007年中医药行业科技专业项目(200707009),国家"重大新药创制"科技重大专项(2009ZX09301-005,2009ZX09308-003) |
| 摘 要： | 目的:采用“一测多评”法与外标法测定新清宁片中4种大黄蒽醌类成分的含量,并对测定结果进行比较.方法:采用RP-HPLC,Aecurasil C18色谱柱(4.6 mm ×250 mm,5 μm),流动相甲醇-0.4％磷酸水溶液(85∶ 15),流速1 mL· min-1,柱温30℃,检测波长254 nm.结果:20批新清宁片中4种蒽醌类成分QAMS计算值与ESM实测值无显著性差异,RSD＜ 5％.结论:在一定色谱条件下,QAMS确定的相对校正因子可作为常数用于含大黄制剂的质量评价. |
| 关 键 词： | "一测多评"法 新清宁片 蒽醌 相对校正因子 相对保留值 外标法 |
| 收稿时间： | 2012/1/29 0:00:00 |
Determination of Rhubarb Anthraquinones in Xinqingning Tablets by QAMS and External Standard Method |
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| ZHANG Kai-bing,FENG Wei-hong,WANG Zhi-min,ZHANG Qi-wei,LI Xi,YANG Fei,LI Dong-ying and JI Li-na.Determination of Rhubarb Anthraquinones in Xinqingning Tablets by QAMS and External Standard Method[J].China Journal of Experimental Traditional Medical Formulae,2012,18(11):61-65. | |
| Authors: | ZHANG Kai-bing FENG Wei-hong WANG Zhi-min ZHANG Qi-wei LI Xi YANG Fei LI Dong-ying and JI Li-na |
| Institution: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Affiliated Pharmaceutical Factory, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
| Abstract: | Objective:To determine the contents of four kinds of anthraquinone components in Xinqingning tablet by quantitative analysis of multi-component with a single-marker(QAMS) and external standard method(ESM) respectively,and compare results by this two methods.Method:RP-HPLC determination was performed at 30 ℃ with Accurasil C18 column(4.6 mm×250 mm,5 μm),mobile phase of methanol-0.4% phosphoric acid(85∶15),detection wavelength at 254 nm,and flow rate of 1.0 mL·min-1.Result:There was no significant difference between calculated value of QAMS and measured value of ESM for four kinds of anthraquinone components from Xinqingning tablet,RSD<5%.Conclusion:Under certain chromatographic conditions,relative correlation factor could be used as a constant for quality evaluation of Rheum palmatum preparation which was determined by QAMS. |
| Keywords: | quantitative analysis of multi-components by single marker Xinqingning tablet anthraquinone relative correlation factor(RCF) relative retention value(RTR) external standard method |
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