初匀速法预测制川乌的有效期

目的:采用化学动力学方法预测制川乌有效期。方法:采用HPLC测定苯甲酰乌头原碱、苯甲酰次乌头原碱、苯甲酰新乌头原碱、乌头碱、次乌头碱、新乌头碱含量,流动相乙腈(A)-40 mmol·L-1乙酸铵溶液(B)梯度洗脱(0~45 min,30%~45%A;45~65 min,45%~50%A;65~75 min,50%~55%A;75~80 min,55%~65%A;80~85 min,65%A),检测波长240 nm。采用初均速法对制川乌药材进行加速试验并预测其有效期,考察不同温度下制川乌药材中双酯型生物碱和单酯型生物碱含量变化情况。结果:制川乌在室温条件下贮存期0.69年。结论:高温不利于制川乌的稳定,初均速法可为制川乌药材有效期的建立提供参考。

Forecast on Shelf Life of Aconiti Radix Cocta by Initial Average Rate Test

Objective: To predict shelf life of Aconiti Radix Cocta and provide basis for assessing its quality. Method: Contents of benzoylaconine,benzoylhypacoitine,benzoylmesaconine,aconitine,hypaconitine and mesaconine were determined by HPLC,with acetonitrile(A)-40 mmol·L^-1 ammonium acetate(B) (0-45 min,30%-45%A;45-65 min,45%-50%A;65-75 min,50%-55%A;75-80 min,55%-65%A;80-85 min,65%A) as mobile phase by gradient eluting,detection wavelength was set at 240 nm.Initial average rate test was adopted to predict shelf life,contents change of diester alkaloids and monoester alkaloids in Aconiti Radix Cocta under different temperature were investigated. Result: Shelf life of Aconiti Radix Cocta had been determined as 0.69 years. Conclusion: High temperature was not conducive to stability of Aconiti Radix Cocta,initial average rate test could provide a reference for establishing validity of herbs.