复杂性疾病维吾尔医分型及其血栓前状态研究

目的 观察不同体液证型患者血栓前状态分子标志物的变化，探讨各体液证型与血栓前状态的关系。方法采用流式细胞术、放射免疫法和ELISA等方法，检测复杂性疾病异常黑胆质证外周血中CD41、CD62p的表达及内皮素（ET-1）、组织型纤溶酶原激活剂及抑制剂（t-PA、PAI）、纤维蛋白原（FIB）、活化部分凝血活酶时间（PT）、凝血酶时间（TT）、凝血酶原时间（APTT）水平，并进行分析。结果复杂性疾病中异常黑胆质证占65．96％，非异常黑胆质证占34．04％。与正常对照组相比，异常黑胆质证组和非异常黑胆质（异常血液质、异常黏液质、异常胆液质）证组CD62p、FIB、PAI、ET-1水平升高（P〈0．05），t-PA水平降低（P〈0．05），PT、APTT时间缩短（P〈0．05）。异常黑胆质证组与异常血液质证组和异常胆液质证组相比，ET-1水平升高（P〈0．05）；与异常黏液质证组和异常胆液质证组相比，CD62p阳性百分数增多（P〈0．05）；与异常血液质证组和异常黏液质证组相比，FIB含量升高（P〈0．05）。结论异常黑胆质证可能是肿瘤、糖尿病、高血压和冠心病等复杂性疾病的维医重要证型。复杂性疾病异常黑胆质证患者血栓前状态改变明显。

Study on Classification of Complex Diseases in Uygur Medicine and Its Prethrombotic State

Objective To observe the pre-thrombosis markers in patients of different body fluids and discuss the relations between body fluids type and prethrombosis state. Methods The expression of CD41, CD62p on platelets, the level of plasma tissue plasminogen activator （t-PA） and its inhibiter （PAI-1）, endothelin （ET-1）, activated partial thromboplastin time （APTT）, fibrinogen （FIB）, prothrombin time （PT） and thrombin time （TT） were tested by using flow cytometer, ELASA, radioimmunoassay method and auto coagulometer. Results Patients with Abnormal Savda syndrome occupy 65.96% and non Abnormal Savda syndrome occupy 34.04% in complex diseases. Compared with normal control group, the average expression of CD62p, the level of plasma PAI-1, ET-1, FIB both in Abnormal Savda syndrome and non Abnormal Savda syndrome groups were significantly increased （P〈0.05）, the level of t-PA was decreased and APTT, PT were significantly shortened （P〈0.05）. Compared with Abnormal Han and Abnormal Sapra syndromes, the level of ET- 1 was significantly increased in Abnormal Savda syndrome （P 〈0.05）. Compared with Abnormal Balham and Abnormal Sapra syndromes, the expression of CD62p was significantly increased in Abnormal Savda syndrome （P 〈0.05）. Compared with abnormal Han and Abnormal Balham syndromes, the level of FIB was significantly increased in Abnormal Savda syndrome （P 〈0.05）. Conclusion Abnormal Savda Syndrome might be an important syndrome type in tumors, diabetes, hypertension and coronary heart diseases in Uygur medicine. Prethrombosis state is more obvious in Abnormal Savda syndrome than that of non Abnormal Savda syndrome.