两种不同提取方法对赤芍中丹皮酚含量的影响

目的建立以高效液相色谱法测定赤芍中丹皮酚含量的方法。方法采用超声和回流两种方法提取赤芍药材中的丹皮酚,采用高效液相色谱法测定其含量。色谱柱为Agilent ZORBAX SB-C18(Stable Bond Analytica l4.6mm×250mm,5μm),流动相为甲醇-水(45∶55),流速为1mL/min,柱温25℃,检测波长274nm。结果线性回归方程为:Y=1.01×105X-9.84×102,r=0.9999(n=5),在0.104～0.52μg范围内丹皮酚的峰面积与进样量有良好的线性关系。结论本法操作简便、准确、重复性好,可用于赤芍药材中丹皮酚含量的测定,同时可作为赤芍药材在炮制前后的质量控制方法之一。

Effect of Two Extracting Methods on Content of Paeonol in Paeonia Iactiflora Pall.by HPLC

Objective To establish a HPLC method for determining the content of Paeonol in Paeonia lactiflora Pall. Methods Ultrasound and reflux methods were used to extract Paeonol in Paeonia lactiflora Pall, and then the content of Paeonol was determined by HPLC. It was performed on Agilent column ZORBAX SB-C18 （Stable Bond Analytical 4.6 minX250 mm, 5 μm）, mobile phase was methanol-water （45 ： 55）, flow rate was 1 mL/min, column temperature was 25 ℃, and detection wavelength was 274 nm. Results The regression equation was Y = 1.01 × 10^5X - 9.84× 10^2, r = 0.999 9 （n = 5）. The standard curve was linear within the range of 0.104-0.52 μg for Paeonol. Conclusion The method is simple and accurate with good reproducibility. It is suitable for determining the content of Paeonol in Paeonia lactiflora Pall. and control the quality control of Paeonia lactiflora Pall. in process.