电针对SAMP8小鼠皮质及海马突触素和突触后致密物-95表达的影响

目的观察电针对SAMP8小鼠皮质及海马突触素(SYN)和突触后致密物-95(PSD-95)表达的影响,探讨电针治疗阿尔茨海默病的介入时机。方法 SAMP8小鼠48只随机分为模型组、3月龄电针组、6月龄电针组和9月龄电针组,每组12只,选取同龄正常老化SAMR1小鼠12只为对照组。电针组电针"百会""大椎""肾俞",频率2 Hz,每日1次,8 d为1个疗程,疗程间隔2 d,共3个疗程。各组均在10月龄取材,选择皮质及海马区,采用免疫组化和Westernblot检测突触相关蛋白SYN、PSD-95的表达。结果与对照组比较,模型组小鼠皮质及海马SYN和PSD-95蛋白表达明显降低(P<0.01);与模型组比较,各电针组小鼠皮质及海马SYN、PSD-95蛋白表达明显升高(P<0.05,P<0.01);与3月龄电针组比较,6月龄电针组、9月龄电针组皮质及海马SYN、PSD-95蛋白表达明显降低(P<0.05,P<0.01)。结论电针可促进SAMP8小鼠皮质及海马SYN、PSD-95蛋白的表达,改善突触功能,且早期电针治疗效果最明显。

Effects of Early Electroacupuncture on Expressions of Synapsin and PSD-95in Cortex and Hippocampus of SAMP8 Mice

Objective To observe the effects of electroacupuncture on the expressions of synaptophysin(SYN)and postsynaptic density-95(PSD-95)in the cortex and hippocampus of SAMP8 mice at different stages;To explore the timing of electroacupuncture in the treatment of Alzheimer disease.Methods Totally 48 SAMP8 mice were randomly divided into model group,3-month-old electroacupuncture group,6-month-old electroacupuncture group and 9-month-old electroacupuncture group,with 12 mice in each group.12 SAMR1 mice of the same age of normal aging were set as control group.“Baihui”(GV20),“Dazhui”(GV14)and“Shenshu”(BL23)acupoints were selected in the electroacupuncture groups.The frequency of electroacupuncture was 2 Hz,once a day,and 8 days was a course of treatment.Samples were collected from each group at the age of 10 months,and the protein expressions of SYN and PSD-95 in cortex and hippocampus were detected by immunohistochemistry and Western blot.Results Compared with the control group,expressions of SYN and PSD-95 protein in the cortex and hippocampus of the model group decreased significantly (P<0.01). Compared with the model group, expressions of SYN and PSD-95 protein in the cortex andhippocampus of mice in each electroacupuncture group significantly increased (P<0.05, P<0.01). Compared with3-month-old electroacupuncture group, the expressions of SYN and PSD-95 protein in the cortex andhippocampus of 6-month-old and 9-month-old electroacupuncture groups decreased significantly (P<0.05, P<0.01).Conclusion Electroacupuncture can promote the expressions of SYN and PSD-95 protein incortex and hippocampusof SAMP8 mice, and improve the synaptic function, and early electroacupuncture has the most obvious efficacy.