| 三七总皂苷对β-淀粉样蛋白42损伤脑微血管内皮细胞ZO-1表达的影响 |
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| 引用本文： | 杨丽鹍,;玛娜璐璐,;朱海燕,;马涛,;孙逸坤,;赵一舟,;高永红.三七总皂苷对β-淀粉样蛋白42损伤脑微血管内皮细胞ZO-1表达的影响[J].中国中医药信息杂志,2014(8):57-59. |
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| 作者姓名： | 杨丽鹍 ;玛娜璐璐 ;朱海燕 ;马涛 ;孙逸坤 ;赵一舟 ;高永红 |
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| 作者单位： | [1]北京市中医药大学东直门医院东区,北京101100; [2]北京中医药大学东直门医院,中医内科学教育部重点实验室和北京市重点实验室,北京100700; [3]北京中医药大学东方医院,北京100078 |
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| 基金项目： | 国家自然科学基金（81173229、81202686）;北京中医药大学实验室开放课题（2011-SYSKFKT-01-2） |
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| 摘 要： | 目的观察三七总皂苷对β-淀粉样蛋白(Aβ)42损伤脑微血管内皮细胞ZO-1表达的影响,为阿尔茨海默病的对因治疗提供新的依据。方法细胞分为正常对照组、模型组和三七总皂苷低、中、高剂量组,37℃、5%CO2培养箱中培养24 h后,采用MTT比色法检测细胞活力,Western blot检测ZO-1蛋白表达。结果 Aβ42刺激后微血管内皮细胞活力降低(P0.01),ZO-1蛋白表达被抑制(P0.01);与模型组比较,三七总皂苷低、中、高剂量组微血管内皮细胞活力增加,其中三七总皂苷中、高剂量组增加较为明显,差异有统计学意义(P0.05),ZO-1蛋白表达增加。结论三七总皂苷通过抑制Aβ42所致微血管内皮细胞活力降低,恢复血脑屏障的部分屏障功能。
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| 关 键 词： | 三七总皂苷 阿尔茨海默病 β-淀粉样蛋白 ZO-蛋白 |
Effects of Panax Notoginseng Saponins on Expression of ZO-1 of Brain Microvascular Endothelial Cells Caused by Aβ42 |
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| Institution: | YANG Li-kun, MANA Lulu, ZHU Hai-yan, MA Tao, SUN Yi-kun, ZHAO Yi-zhou, GAO Yong-hong (1.Dongzhimei Eastern Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 101100, China; 2.Key Laboratory of Chinese Internal Medicine Under Ministry of Education and Beijing, Dongzhimeng Hospital Beijing University of Chinese Medicine, Beijing 100700, China 3.Dong)Cang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China) |
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| Abstract: | Objective To study the effects of Panax Notoginseng Saponins （PNS） on the damaged expression of ZO-1 of brain microvascular endothelial cells caused by Aβ42.MethodsBrain microvascular endothelial cells were divided into normal control group, model group, PNS low-, medium- and high concentration groups. They were incubated for 24h in 5% CO2 incubator at 37℃ . Then cell vitality was detected by MTT colorimetric method and ZO-1 protein expression was tested by Western blot.Results Stimulation of Aβ42 reduced the activity of microvascular endothelial cells （P〈0.01） and suppressed the expression of ZO-1 protein （P〈0.01）. Compared with the model group, the activity of microvascular endothelial cells of PNS groups, especially the high and medium dose groups （P〈0.05）, and increased the ZO-1 protein expression. Conclusion PNS can partly recover the barrier function of blood brain barrier through inhibiting the decrease of the activity of microvascular endothelial cells caused by Aβ42. |
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| Keywords: | Panax Notoginseng Saponins Alzheimer＇s disease β-amyloid42 ZO-1 |
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