银杏酮酯对小鼠学习记忆能力及海马神经炎症反应的影响

目的观察银杏酮酯对自然衰老小鼠学习记忆能力和海马神经炎症反应的影响,探讨银杏酮酯抗衰老的作用机制。方法 16月龄ICR小鼠随机分为模型组和银杏酮酯高、低剂量组,1月龄ICR小鼠作为正常组。其中银杏酮酯高、低剂量组给予不同剂量的银杏酮酯灌胃,模型组和正常组给予1%羧甲基纤维素钠灌胃。采用Morris水迷宫和跳台实验进行行为学检测,免疫组织荧光化学方法检测海马离子钙接头蛋白抗原(Iba-1)数量,酶联免疫吸附试验测定海马肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)表达。结果与正常组比较,模型组Morris水迷宫的逃避潜伏期和游泳距离均延长(P0.05),跳台实验中潜伏期缩短和错误次数增加(P0.05),海马Iba-1阳性细胞数增加(P0.01),TNF-α表达有上调趋势,IL-1β表达上调(P0.01);与模型组比较,银杏酮酯高剂量组的逃避潜伏期和游泳距离有降低趋势,跳台实验的潜伏期延长和错误次数降低(P0.05),海马Iba-1阳性细胞数减少(P0.05);银杏酮酯低剂量组TNF-α表达有下降趋势,IL-1β表达下降显著(P0.01)。结论银杏酮酯能增强自然衰老小鼠学习记忆能力,延缓衰老发生。

Effects of Ginkgo Biloba Extract on Learning Memory Ability and Inflammatory Response in Hippocampus of Natural Aging Mice

Objective To evaluate the efficiency of Ginkgo biloba extract 50 （GBE50） on learning memory ability and inflammatory response in hippocampus of natural aging mice, and explore the underlying anti-aging mechanisms.Methods 16-month-old ICR mice were randomly divided into three groups：model group, GBE50 low and high dose groups. 1 month mice were as normal group. The mice in GBE50 low and high dose groups received GBE50. The mice in the normal and model groups received solvent （1%CMC-Na＋） by intragastric administration. The Morris water maze test and step-down test were used to assess behaviors of the mice. Immunofluorescent staining was used to detect the number of Iba-1 positive cells. The enzyme linked immunosorbent assay was used to determine the expression of TNF-α and IL-1β.Results Compared with normal group, escape latency and swimming distance in the Morris water maze test in the model group increased （P〈0.05）;latency shorted and error times decreased in the step-down test （P〈0.05）;the number of Iba-1 positive cell in the hippocampus CA1 in the model group increased considerably （P〈0.01）;TNF-α expression was in a general upward trend while IL-1β increased apparently （P〈0.01）. Compared with model group, escape latency and swimming distance decreased in the Morris water maze test in GBE50 high dose group;latency and the error times increased in the step-down test （P〈0.05）;the number of Iba-1 positive cells decreased （P〈0.05）. TNF-α expression showed a downward trend and IL-1β expression decreased in GBE50 low dose group （P〈0.05）. Conclusion GBE50 can delay aging and increase learning memory of natural aging mice.