| 复合酶辅助超声波提取沙枣多糖的工艺研究 |
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| 引用本文： | 廉宜君,李炳奇,刘红,马彦梅,陈韩英.复合酶辅助超声波提取沙枣多糖的工艺研究[J].中国中医药信息杂志,2009,16(6):47-48. |
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| 作者姓名： | 廉宜君 李炳奇 刘红 马彦梅 陈韩英 |
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| 作者单位： | 1. 石河子大学化学化工学院,新疆,石河子,832000
2. 石河子大学药学院,新疆,石河子,832000 |
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| 基金项目： | 石河子大学高层次人才科研启动资金专项基金 |
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| 摘 要： | 目的研究复合酶辅助超声波提取沙枣多糖的最佳工艺。方法考察了纤维素酶和果胶酶在不同温度、不同酶量、不同酶作用时间下提取沙枣多糖最佳的方案，并且研究了超声处理对于酶法提取沙枣多糖的影响。结果沙枣多糖提取的适宜条件是：纤维素酶1.5％、果胶酶1．5％；酶解时间为40min，酶解温度为55℃，超声时间25min，超声功率400W，固液比1：30，超声温度60℃。在此条件下，沙枣多糖的提取率可达12．35％。结论本工艺简单可行，可用于沙枣多糖的提取。
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| 关 键 词： | 沙枣 多糖 酶 超声波 提取工艺 |
Study on Extraction Process of Polysaccharides from Elaeagnus angustifolia L.by Enzymatic-assisted Ultrasonic Methods |
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| Institution: | LIAN Yi-jun, LI Bing-qi, LIU Hong, et al (College of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832000, China) |
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| Abstract: | Objective To optimize the technological conditions of polysaccharide extraction from Elaeagnus angustifolia L. by enzymatic-assisted ultrasonic. Methods The effects of different enzymes, enzymolysis temperature, enzyme dosage, enzymolysis time, material-water ratio, crushing degree, ultrasonic time and ultrasonic temperature on the extraction rate of Elaeagnus angustifolia L. polysaccharide was analyzed. Results The optimal technological condition was as follows： 1.5% cellulase, 1.5% pectic enzyme, enzymolysis time 40 min, enzymolysis temperature 55℃, ultrasonic time 25 min, ultrasonic power 400 W, material- water ratio 1 ： 30, and temperature 60 ℃. In these conditions, the extraction efficiency of polysaccharide was 12.35%. Conclusion The process is simple and practicable, and can be used for the extraction of Elaeagnus angustifolia L. polysaccharide. |
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| Keywords: | Elaeagnus angustifolia L polysaccharides enzyme ultrasonic extraction process |
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