| HPLC同时测定尿塞通胶囊中丹参素钠、橙皮苷及丹酚酸B含量 |
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| 引用本文： | 王绍青,金星,张清波.HPLC同时测定尿塞通胶囊中丹参素钠、橙皮苷及丹酚酸B含量[J].中国中医药信息杂志,2014(6):83-85. |
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| 作者姓名： | 王绍青 金星 张清波 |
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| 作者单位： | [1]黑龙江中医药大学,黑龙江哈尔滨150040; [2]黑龙江省食品药品检验检测所,黑龙江哈尔滨150001 |
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| 基金项目： | 2011年国家药品标准提高行动计划（595） |
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| 摘 要： | 目的建立同时测定尿塞通胶囊中丹参素钠、橙皮苷、丹酚酸B含量的方法。方法采用高效液相色谱法,C18色谱柱,以乙腈-0.1%磷酸为流动相进行梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长286 nm。结果丹参素钠、橙皮苷、丹酚酸B分别在0.038 44~1.922μg、0.000 207 052 8~4.044μg、0.034 28~1.714μg范围内线性关系良好,平均回收率为90.9%~107.1%,RSD为2.1%~3.2%。结论所建立的方法简便、准确,可用于尿塞通胶囊的质量控制。
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| 关 键 词： | 尿塞通胶囊 丹参素钠 橙皮苷 丹酚酸B 高效液相色谱法 含量测定 |
HPLC Simultaneous Determination of Sodium Danshensu,Hesperidin and Salvianolic ;Acid B in Niaosaitong Capsules |
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| Institution: | WANG Shao-qing, JIN Xing, ZHANG Qing-bo (1.Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China. 2.Heilongiiang Institute for Food and Drug Control Harbin 150001, China) |
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| Abstract: | Objectives To establish an HPLC method for determination of sodium Danshensu, hesperidin and salvianolic acid B in Niaosaitong capsules. Methods Three components were separated by C18 chromatographic column with a gradient mobile phase consisting of acetonitrile and 0.1%phosphoric acid solution at a flow rate of 1.0 mL/min, column temperature of 30 ℃, and detection wavelength was set at 286 nm. Results Sodium Danshensu, hesperidin and salvianolic acid B had good linearity in the range of 0.038 44-1.922 μg, 0.000 207 052 8-4.044 μg, 0.034 28-1.714 μg respectively, and the average recovery rate was 90.9%-107.1%, RSD was 2.1%-3.2%. Conclusion The methods is simple and accurate, and can be used for the quality control of Niaosaitong capsules. |
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| Keywords: | 丹酚酸B Niaosaitong Capsules sodium Danshensu hesperidin salvianolic acid B HPLC content determination |
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