红景天对血管性痴呆大鼠行为学及海马CA1区Bcl-2及Bax表达的影响

目的观察红景天对血管性痴呆大鼠认知功能及海马CA1区Bcl-2、Bax蛋白表达的影响，探讨红景天的神经保护作用。方法采用双侧颈总动脉永久结扎法制备前脑缺血致血管性痴呆模型，以MorriS水迷宫检测大鼠学习记忆能力；以免疫组化检测大鼠海马CA1区Bcl-2、Bax蛋白表达。结果造模组与对照组相比，认知能力明显下降（P〈0．01），Bcl—2、Bax蛋白表达增加（P〈0．01）；治疗组与模型组相比，认知能力明显改善（P〈0．01），Bcl-2蛋白表达明显增加（P〈0．01），Bax蛋白表达明显减少（P〈0．01）。结论血管性痴呆大鼠给予红景天后，可以通过调节海马Bcl-2及Bax蛋白表达，抑制神经细胞凋亡，保护神经细胞，促进脑功能的恢复，从而改善大鼠学习记忆能力。

Effects of Rhodiola Herb on Behavior and the Expression of Bcl-2 and Bax Protein in the Hippocampus of Vascular Dementia Rats

Objective To study the effects of Rhodiola herb on behavior and the expression of Bcl-2 and Bax protein in hippocamps of vascular dementia rat induced by chonic cerebral ischemia. And the protective effect of Rhodiola herb was studied. Methods VD model was formed by adopting the permanent occlusion of bilateral carotid arteries in Wistar rats to produce the forebraln ischemia. Cognitive ability of rats were studied with the Morris water maze. The expression of Bcl-2 and Bax protein in the hippocampus were determined with immunohistochemical staining. Results VD model may decrease cognitive ability （P〈0.01）, increase the expression of Bcl-2 and Bax protein in the hippocampus （P〈0.01）. Rhodiola herb alleviated cognitive ability significantly （P〈0.01）, increased the expression of Bcl-2 protein （P〈0.01） and reduced the expression of Bax protein （P〈0.01）. Conclusion Rhodiola herb can inhibit apoptosis through regulating the expression of Bcl-2 and Bax protein in the hippocampus. Rhodiola herb may improve cognitive ability in Vascular Dementia rats.