青钱柳等几种别样茶中咖啡因、可可碱和茶碱的检测

目的：对青钱柳甜茶等几种别样茶中咖啡因、可可碱和茶碱进行检测，为别样茶的合理应用奠定基础。方法：采用超快速液相色谱法（RSLC），AcclaimPA2色谱柱（150mm×2．1mm，2．2μm），乙腈-水梯度洗脱；流速：0．5mL·min-1；检测波长：273nm，柱温：30℃。结果：除加入绿茶的商品中检测到适量的咖啡因外，青钱柳神茶、黄芩茶、多穗柯甜茶、悬钩子属甜茶，以及甜叶菊叶和湖北海棠叶中均未检测出上述3种生物碱。结论：相对于绿茶中含有较高含量的咖啡因、可可碱和茶碱，别样茶中未检出上述3种成分，为别样茶的科学应用提供指导。

Determination of Caffeine, Theobromine, and Theophylline in the Leaves of Cyclocarya paliurus and Several Other Kinds of Tea

Objective： To determine caffeine, theobromine, and theophylline contents in the leaves of Cyclocarya paliurus and several other kinds of tea by RSLC. Methods：The separation was carried out through a gradient elution using a Dionex Acclaim RSLC PA2 （150 mm ×2.1mm,2.2μm） column and a mobile phase consisting of water and aeetonitrile at a flow rate of 0. 5 mL. min - 1. The detection wavelength was set at 273 nm. The column temperature was 30 ℃. Results：Under the optimized conditions, all the 3 analytes were successfully separated within 10 min ,and good linearity（ r 〉0. 999 6）was achieved. This method was also validated with respect to precision, specificity, and was successfully applied to simultaneous determination of 3 compounds in the leaves of Cyclocarya paliurus and several other kinds of tea including： Huang-qin tea, Duo-sui-ke tea, Xuan-gou-zi sweet tea, Tian-ye-ju tea, Hubei-hai-tang tea. The results indicate that the 3 compounds were not detected in the six species of plants. Conclusion：It can guide the application of science for other kinds of tea.