| 针刺干预对脑缺血再灌注损伤大鼠外周血清蛋白表达的影响 |
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| 引用本文： | 陈素辉,孙华,徐虹,黄艳秋,高扬.针刺干预对脑缺血再灌注损伤大鼠外周血清蛋白表达的影响[J].针灸临床杂志,2011,27(9):50-53. |
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| 作者姓名： | 陈素辉 孙华 徐虹 黄艳秋 高扬 |
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| 作者单位： | 1. 中国医学科学院北京协和医学院北京协和医院,北京,100730
2. 中国医学科学院北京协和医学院基础医学研究所,北京,100005 |
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| 摘 要： | 目的:探讨脑缺血再灌注损伤模型大鼠外周血清蛋白表达的变化及针刺百会、足三里穴的干预作用。方法:以大脑中动脉线栓法制备大鼠脑缺血再灌注模型,采用随机区组设计将大鼠分为模型组、假手术组和针刺组3个大组,每大组再按照再灌注后时间分成12 h、24 h、48 h、72 h、96 h和144 h等6个亚组;每个亚组有大鼠7只;另设正常对照组7只。应用SELD I-TOF-MS检测各组大鼠外周血清蛋白表达的差异。结果:模型组与正常对照组比较,有150个差异蛋白峰(P<0.05);针刺组与模型组结果比较,有89个差异蛋白峰出现显著性变化,包括正向峰32个和负向峰57个;模型组血清蛋白峰值的分布呈双峰型,针刺干预使第一峰峰值降低,第二峰峰值时间前移。结论:针刺百会和足三里穴能够通过影响脑缺血再灌注损伤大鼠外周血清蛋白的表达,干预缺血再灌注模型大鼠的病理生理过程。
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| 关 键 词： | 针刺 百会穴 足三里穴 脑缺血再灌注损伤 表面增强激光解吸电离飞行时间质谱 |
Effect of Acupuncture on the Expression of Peripheral Serum Protein in Rats with Cerebral Ischemia Reperfusion Injury |
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| CHEN Su-hui,SUN Hua,XU Hong,HUANG Yan-qiu,GAO Yang.Effect of Acupuncture on the Expression of Peripheral Serum Protein in Rats with Cerebral Ischemia Reperfusion Injury[J].Journal of Clinical Acupuncture and Moxibustion,2011,27(9):50-53. |
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| Authors: | CHEN Su-hui SUN Hua XU Hong HUANG Yan-qiu GAO Yang |
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| Institution: | CHEN Su-hui1,SUN Hua1,XU Hong1,HUANG Yan-qiu2,GAO Yang2 (1.Peking Union Medical College Hospital,Beijing 100730,China,2.Institute of Basic Medical Sciences,Beijing 100005,China) |
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| Abstract: | Objective:To explore the expression of peripheral serum protein expression in cerebral ischemia/reperfusion injury(CI/RI) rats and the influence of acupuncture points of Baihui(DU20) and Zusanli(ST36)on it.Methods:CI/RI model was established by using right middle cerebral artery occlusion and reperfusion.SD rats were randomly divided into model group,sham-operation group and acupuncture group,each group was further divided into 12 h,24 h,48 h,72 h,96 h and 144 h six subgroups(7 rats in each subgroup),adding... |
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| Keywords: | Acupuncture Baihui Zusanli Cerebral ischemia-reperfusion injury SELDI-TOF-MS |
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