痰热清注射液抗内毒素所致急性肺损伤的实验研究

 目的探讨痰热清注射液抗内毒素所致急性肺损伤的作用机制。方法取体重180-220g的健康清洁级SD大鼠72只,雌雄各半,随机分为正常组、模型对照组、甲基泼尼松龙组、清开灵注射液组、痰热清注射液小剂量组、痰热清注射液大剂量组,每组12只。模型制作采用舌下静脉注射大肠杆菌内毒素(LPS,Ecoli05585)6mg·kg^-1致急性肺损伤。观察腹腔注射给药1次8h后的肺含水量、肺泡灌洗液蛋白与细胞数、动脉血氧分压、血氧饱和度,并结合病理学观察分析诸项指标变化的意义。结果痰热清、清开灵和甲基泼尼松龙能够有效阻抑LPS导致的肺含水量及肺泡灌洗液蛋白含量增加,痰热清还能够显著减少肺泡灌洗液的细胞数量和TNF-α含量,阻抑肺损伤导致的动脉血氧分压和氧饱和度的下降。其减轻肺泡壁结构损伤、肺间质水肿,改善肺泡壁毛细血管淤血状态的作用显著优于清开灵和甲基泼尼松龙。结论痰热清注射液能够在阻抑内毒素导致的炎症级联反应的同时,改善肺泡壁毛细血管血流状态,是其有效减轻急性肺损伤,阻止动脉血氧分压和氧饱和度下降的药理学基础。

Protective effects of Tanreqing on lipopolysaccharide-induced acute lung injury in rats

OBJECTIVE To study the effects of Tanreqing parenteral solution on acute lung injury induced by lipopolysaccharide. METHODS Seventy-two healthy Sprague-Dawley rats weighing 180 - 220 g were randomly divided into six groups: normal group, model group, methylprednisolone-treatment group, Qingkailing-treatment group, Tanreqing-treatment group (common dosage), Tanreqing-treatment group(large dosage). Each group included twelve animals. LPS(Ecoli055B5) was injected through sublingual vein(6 mg·kg^-1) to induce a-cute lung injury. The changes of water content of lung were studied, BALF( bronchoalceolar lavage fluid) protein and the number of cells were examined. Partial pressures of oxygen and oxygen saturation degree of artery blood were measured, and the pathologic change of morphology was observed too. RESULTS Tanreqing and Qingkailing as well as methylprednisolone effectively downregulated the increasing of water content of lung and BALF protein. Tanreqing dramatically inhibited the increase of TNF-α content and cells number in BALF,and inhibited the decrease of partial pressure of oxygen and oxygen saturation degree in artery blood, which caused by lung injury. In particular, it could relieved the injury of alveolar wall and the progress of pulmonary interstitial edema and ameliorated the stasis of blood capillary of alveolar wall, which was evidently better than the effects of Qingkailing and methylprednisolone. CONCLUSION Tanreqing had the protective effects of relieving acute lung injury and inhibiting the decrease of partial pressure of oxygen and oxygen saturation degree of artery blood, which was partially attributed to the inhibition of cascade of response of inflammation caused by LPS and the amelioration of circulation of blood capillary of alveolar wall.