蜂胶总黄酮对自发性糖尿病大鼠糖脂代谢的影响及抗氧化作用

目的研究蜂胶总黄酮(TFP)对自发性糖尿病大鼠糖脂代谢的影响。方法将50只自发性糖尿病大鼠按血糖值随机分为对照组、消渴丸组(2 000 mg·kg-1)、蜂胶总黄酮高剂量组(240 mg·kg-1)、中剂量组(120 mg·kg-1)、低剂量组(60mg·kg-1)。各组大鼠每天灌胃给药1次,连续8周,对照组灌胃同体积羧甲基纤维素钠溶液。末次给药结束后,禁食12 h,乙醚麻醉,腹主动脉取血,检测自发性糖尿病大鼠血清中血糖(Glu)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、糖化血红蛋白(GHb)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)、胰岛素(INS)、C肽(C-P)、肿瘤坏死因子(TNF-α)、游离脂肪酸(FFA)、一氧化氮(NO)及肝糖原水平。结果与对照组比较,蜂胶总黄酮高、中、低剂量组血糖、甘油三酯、总胆固醇、低密度脂蛋白胆固醇、丙二醛及肿瘤坏死因子-α水平明显降低(P<0.05或P<0.01或P<0.001),高密度脂蛋白胆固醇、超氧化物歧化酶、胰岛素、C肽及肝糖原含量明显升高(P<0.05或P<0.01或P<0.001);蜂胶总黄酮高、中剂量组糖化血红蛋白及游离脂肪酸水平明显降低(P<0.05或P<0.01或P<0.001),谷胱甘肽及一氧化氮含量明显升高(P<0.05或P<0.01),低剂量组则无显著性差异(P>0.05)。消渴丸组超氧化物歧化酶、肝糖原、一氧化氮及C肽明显升高(P<0.05或P<0.01),血糖、丙二醛、甘油三酯及糖化血红蛋白水平明显降低(P<0.05或P<0.01或P<0.001),对其他指标则无明显影响(P>0.05)。结论蜂胶总黄酮可明显降低自发性糖尿病大鼠血糖水平,明显改善自发性糖尿病大鼠糖脂代谢及抑制胰岛素抵抗。

Glucolipid Metabolism and Antioxidant Effect of the Total Flavones of Propolis in GK Rats

OBJECTIVE To study on glucolipid metabolism effect of the total flavones of propolis(TFP) in Goto-Kakisaki rats(GK Rats) . METHODS Fifty GK rats were randomly divided into 5 groups by blood glucose, the model group, positive drug group(2 000 mg·kg^-1) and the high(240 mg·kg^-1) dose of the TFP group, the medium(120 mg·kg^-1) doses of the TFP group, low(60 mg·kg^-1) doses of the TFP group. The GK rats in TFP groups were given with TFP by intragastric administration, continuously treated for 8 weeks, the control group were given equal sodium carboxymethylcellulose(CMC-Na) and positive drug group were given(2 000 mg·kg^-1) dose of Xiaoke pills by intragastric administration. The glucose(Glu), total cholesterol(TC),triglyceride(TG), high density lipoprotein(HDL-C), low density lipoprotein(LDL-C), glycated hemoglobin(GHb), superoxide dismutase(SOD), malondialdehyde(MDA), glutathione(GSH), serum insulin(INS), C-peptide(C-P), tumor necrosis factor-α(TNF-α) free fatty acid(FFA), nitric oxide(NO), and hepatic glycogen were measured at the end of the experiment. RESULTS Compared with the control group, the levels of GLU, TG, TC, LDL-C,GHb, MDA and TNF-α were decreased significantly(P<0.05 or P<0.01 or P<0.001), the levels of HDL-C,SOD, INS, hepatic glycogen and C-P were increased significantly in the high-dose group, medium-dose group and low-dose group(P<0.05 or P<0.01 or P<0.001). The levels of GHb and FFA were decreased significantly(P<0.05 or P<0.01 or P<0.001), the levels of GSH and NO were increased significantly(P<0.05 or P<0.01) in the high-dose group and medium-dose group, the other indexes had no significant difference(P>0.05) in the low-dose group. The levels of SOD, hepatic glycogen, NO and C-P were increased significantly(P<0.05 or P<0.01), the levels of GLU, MDA, TG and GHb were decreased significantly(P<0.05 or P<0.01 or P<0.001) in Xiaoke pills group. CONCLUSION TFP can significantly decrease the level of blood glucose, significantly improve the glucose and lipid metabolism and inhibit insulin resistance in deficiency of GK rats.