人GPx1基因的克隆及其序列分析

 目的克隆中国人谷胱甘肽过氧化物酶(GPx1)的cDNA。方法用逆转录 聚合酶链反应(RT-PCR)，以中国人胎盘组织总RNA为模板，扩增中国人谷胱甘肽过氧化物酶(GPx1)的cDNA，进行序列分析。计算机分析其二级结构并比较已发现的人GPx家族的5种GPx氨基酸序列。结果从中国人胎盘组织中克隆的GPx1基因，与国外文献报道相比，只有1个氨基酸残基发生变异，即leu²²变为Val²²，该变化不影响GPx1活性中心的结构；人GPx的硒结合区及活性中心区域的氨基酸序列是高度保守的。结论首次克隆了中国人体特异的胞质内GPx1基因，为其生物学活性及结构与功能的关系的研究创造了条件。

Cloning and sequence analysis of human glutathione peroxidase 1

OBJECTIVE To clone the cDNA of human glutathione peroxidase 1(GPx1).METHODS The total RNA was isolated from normal Chinese human placenta and was amplified as the template by RT-PCR.It was inserted into pSK plasmid to analyze its sequence;the sequences of 5 known GPx gene were compared. RESULTS The GPx1 cDNA from placenta had 1 base pare and 1 amino acid residue (Leu²²mutated to Val²²),different from that of reported.But it didn't alter the secondly structure of GPx selenocysteint residue and the active sites were highly conserved in 5 GPxs. CONCLUSION The human GPx1 gene of Chinese was cloned for the first time.