5种海洋动物多糖对体外小鼠脾淋巴细胞及大鼠睾丸支持细胞增殖作用的比较

 目的研究脉红螺、隔贻贝、角蝾螺、福氏玉螺、纵条肌海葵5种海洋动物粗多糖对体外小鼠脾淋巴细胞转化率和大鼠睾丸支持细胞增殖率的作用。方法5种粗多糖分别以100,1 000 mg·L^-1两个浓度组,体外对小鼠脾淋巴细胞和大鼠睾丸支细胞作用72 h后,用MTT法对小鼠脾淋巴细胞的转化率和大鼠睾丸支持细胞的增殖率进行测定。结果角蝾螺(P<0.05),玉螺(低浓度组P<0.05;高浓度组P<0.001)、海葵(P<0.001)多糖能显著提高小鼠的脾淋巴细胞转化率和大鼠睾丸支持细胞的增殖率。结论角蝾螺、玉螺、海葵粗多糖有促进体外小鼠脾淋巴细胞转化和大鼠睾丸支持细胞增殖的作用。

Effects of Polysaccharides from Five Kinds of Marine Animals on Transformation Rate of Mouse Lymphocytes and Multiplication Rate of Rat Stertoli Cells in vitro

OBJECTIVE To study the effect of crude polysaccharides which were extracted from Rapana venosa,Septifer bilocularis,Turbo cornutus,Natica fortunei and Haliplanella luciae on the transformation rate of mouse lymphocytes and the multiplication rate of rat stertoli cells in vitro.METHODS The transformation rate of mouse lymphocytes and the multiplication rate of rat stertoli cells in vitro were tested with MTT method after the cells were cultured with 100 and 1 000 mg·L^-1polysaccharides from five kinds of marine animals respectively.RESULTS The results showed that the polysaccharides from Turbo cornutus(P<0.05),Natica fortunei(low concentration group P<0.05;high concentration group P<0.001),Haliplanella luciae(P<0.001) apparently enhanced the transformation rate of mouse lymphocytes and the multiplication rate of rat stertoli cells in vitro.CONCLUSION The crude polysaccharides from Natica fortunei,Haliplanella luciae and Turbo cornutus can enhance the transformation rate of mouse lymphocytes and the multiplication rate of rat stertoli cells in vitro.