甲钴胺、格列齐特及其联合用药对糖尿病周围神经病变大鼠坐骨神经功能和多元醇代谢通路的影响

 目的　研究甲钴胺、格列齐特及其联合用药对链脲佐菌素(STZ)糖尿病大鼠坐骨神经功能和多元醇代谢通路的影响。方法　动物灌胃给药,连续8周,观察药物对链脲佐菌素糖尿病大鼠坐骨神经形态学改变、运动神经传导速度、坐骨神经Na⁺,K⁺-ATP酶和醛糖还原酶活性及山梨醇、肌醇、果糖、葡萄糖、甘露糖等多元醇含量的影响。结果　格列齐特及格列齐特与甲钴胺联合用药对链脲佐菌素糖尿病大鼠坐骨神经形态学改变有明显保护作用 ,坐骨神经传导速度明显提高 ,坐骨神经Na⁺,K⁺-ATP酶活性增强,醛糖还原酶活性降低,葡萄糖含量减少,联合用药组山梨醇和果糖含量也明显降低。甲钴胺、格列齐特联合用药与两药单用相比,给药两周时的坐骨神经传导速度明显快于格列齐特组,给药8周时坐骨神经中的山梨醇含量较两药单用有非常显著性降低,果糖含量较格列齐特组有明显降低。结论　甲钴胺、格列齐特及其联合用药对STZ糖尿病大鼠周围神经功能有明显的保护作用,对周围神经组织多元醇通路增强有明显的抑制作用,两药合用显示出一定的疗效增强作用。

Effects of methylcobalamin,gliclazide and their combination on peripheral nerve function and polyol pathway in streptozotocin diabetic rats

OBJECTIVE To investigate the effects of methylcobalamin,gliclazide and their combination on peripheral nerve function and polyol pathway in streptozotocin diabetic rats.METHODS The diabetic rats induced by streptozotocin intraperitoneal injection were administerted orally for 8 weeks.Effects of drugs on sciatic nerve morphological alterations,motor nerve conduction velocity,Na ,K ATPase activity and aldose reductase activity,sciatic nerve polyol contents were determined respectively.RESULTS Compared with diabetic group,the sciatic nerve morphological alterations was improved,sciatic nerve conduction velocity increased,aldose reductase activity depressed and Na ,K ATPase activity increased in each group,respectively.The sorbitol and fructose contents were decreased in combination group.The glucose contents were reduced in gliclazide and combination groups.CONCLUSION Methylcobalamin,gliclazide and their combination improve metabolism and function of peripheral nerve in streptozotocin diabetic rats.Synergistic action on polyol pathway was observed in drug combination group.