橙皮苷对羟自由基的清除作用

 目的：探讨了橙皮苷对羟自由基(·OH)的清除作用及其对脂质过氧化的阻抑作用。方法：以·OH清除剂(甘露醇、苯甲酸)为对照，应用Fenton反应检测橙皮苷对·OH的清除作用，并用荧光法测定丙二醛(MDA)研究橙皮苷对红细胞膜脂质过氧化的阻移作用。结果：对·O&20除作用以清除50%·OH所需的药物量(EC₅₀)表示，橙皮苷、甲基橙皮苷、甘露醇和苯甲酸的EC50依次为14，1 800，4 100和500μg。对红细胞膜脂过氧化的抑制率，橙皮苷为68.9％而甘露醇为39，3％。结论；橙皮苷对·OH有明显的清除作用，且呈剂量依赖关系；对由·OH引起的红细胞膜脂过氧化也有明显的抑制。与甲基橙皮苷的作用相比，提示橙皮苷对·OH清除作用与其分子中的酚羟基有关。

Scavenging action to hydroxyl free radical of hesperidin

OBJECTIVE:To discuss the effect of hesperidin scavengingting hydroxyl free radicals and inhibiting lipid peroxidation.METHOD:The scavenging action to hydroxyl free radicals of hesperidin was tested by Fenton reaction contrasting with special eliminations for hydroxyl free radicals(e.g.mannitll,benaoicacid),and the inhibition to lipid peroxidation of erythrocyte ghost of hesperidin was studied by the fluorescent methods for testing the con-tent of malondialdehyde(MDA).RESULTS:The scavenging action to hydroxyl free redicals was represented by the content scavenging 50％of all hydroxyl free radicals (EC₅₀)The EC₅₀ of hesperidin,methyl-hesperidin,mannitll and benzoic acid was 14,1 800,4 100 and 500μg respectively.The incidence of inhibition og hesperidin to lipid peroxidation of erythrocyte ghost was 68.9％,and that of mannitol was 39.3％.CONCLUSION:Hesperidin can apparently scavenge hydroxyl free rdicals connecting with its content,And it can also apparently inhibit the lipid peroxidation of erythrocyte that the scavenging effect of hesperidin on hydroxyl free radicals be connected with phenol hy-droxyls in their molecules.