小续命汤有效成分组对局灶性脑缺血大鼠的作用

 目的 评价小续命汤有效成分组对局灶性脑缺血大鼠的保护作用。方法 采用线栓法阻塞大鼠大脑中动脉建立局灶性脑缺血动物模型。通过神经症状评分和倾斜板实验评价大鼠行为学的改变；TTC染色法观察脑缺血范围；分光光度法测定与脑组织一氧化氮合酶（NOS）活性、丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性及谷胱甘肽过氧化物酶（GSH-px）活性。结果 与局灶性脑缺血模型组相比较，小续命汤有效成分组可显著改善神经症状障碍，延长倾斜板停留时间，减少脑梗死体积，改善局灶性脑缺血引起的脑组织中MDA含量升高、SOD活性降低及NOS活性增高，但对GSH-px无显著影响。结论 小续命汤有效成分组对局灶性脑缺血具有保护作用，其作用机制可能与调节脑内氧化-抗氧化平衡及降低iNOS活性有关。

Effects of the Effective Components Group of Xiaoxuming Decoction on MCAO Rats

OBJECTIVE To investigate the protective effects of the effective components group of Xiao-Xu-Ming decoction(XXM) on focal cerebral ischemia rats.METHODS The model of focal cerebral ischemia was made by middle cerebral artery occlusion(MCAO) with nylon suture.The neuroethological score and the staying time on slope board were gotten after 48 h of the operation.The percentage of cerebral infarction volume to total brain volume was calculated by TTC staining.Furthermore,the content of MDA,the activities of SOD and GSH-Px,and the activity of NOS in the brain tissue were detected by photospectrometer analysis system.RESULTS The effective components group of Xiao-Xu-Ming decoction could significantly reduce the scores of neurological deficits,alleviate the cerebral infarction volume,improve the activity of SOD,reduce the content of MDA,and decrease the activity of iNOS,but have no effect on the activity of GSH-Px.CONCLUSION The effective components group of Xiao-Xu-Ming decoction may attenuate the focal cerebral ischemia injury in rats,and the mechanism is related to regulate the balance between oxidation and anti-oxidation and decrease the activity of iNOS in the brain tissue.