高效液相色谱法同时测定复方氧氟沙星滴耳液中3种组分的含量

 目的：建立HPLC法同时测定复方氧氟沙星滴耳液中氧氟沙星、替硝唑和地塞米松磷酸钠的含量。方法：以氯霉素为内标，ODS为固定相，0.2%三乙胺水溶液(用磷酸调pH至2.70)-乙腈(68∶32)为流动相，检测波长为240 nm。结果：平均回收率及RSD分别为氧氟沙星99.5%，0.8%；替硝唑101.0%，1.2%；地塞米松磷酸钠98.2%，1.5%。结论：本法简便、快速，结果准确，适用于该制剂的质量分析检验。

Simultaneous HPLC determination of 3 components of compound ofloxacin ear drops

OBJECTIVE To establish a method for determination ofloxacin,tinidacole and dexamethason sodium phosphate in compound of loxacin ear drops.METHODS ODS as stationary phase,0.2% triethylamine in water(adjusted to pH2.70 with phosphoric acid)-acetonitrile(68∶32) as mobile phase,chloramphenicol as internal standard,the wavelength of detection was 240nm.RESULTS The average recoveries of ofloxacin,tinidazole and dexamethasone sodium phosphate were 99.5%(RSD=0.8%), 101.0%(RSD=1.2%) and 98.2%(RSD=1.5%)respectively.CONCLUSIONS This method is simple,rapid and accurate for quantitative analysis of the preparation.