阿诺宁乳化剂的抗瘤作用和急性毒性试验

 目的研究阿诺宁乳化剂的抗瘤作用和急性毒性反应。方法用小鼠和裸鼠移植性肿瘤模型测定体内抗瘤活性，用Blliss方法计算小鼠给药的急性毒性的LD₅₀。结果阿诺宁乳化剂在4，8和16 mg·kg^-1，灌胃(ig)qd×10 d，对小鼠肝癌HepS 3批实验的平均抑瘤率分别为39.2%，46.9%和55.7%；对小鼠肉瘤S-180的平均抑瘤率分别为34.6%，47.3%和54.4%；对小鼠L₂的平均抑瘤率分别为37.5%，45.9%和56.5%。阿诺宁乳化剂在7.5，15，30和60 μg·kg^-1腹腔注射(ip)×10d 剂量下，对小鼠肝癌(HepS)实验的平均抑瘤率分别为31.0%，42.9%，50.2%和62.4%；对小鼠肉瘤S-180的平均抑瘤率依次为23.6%，39.4%，50.9%和61.2%；在15，30和60μg·kg^-1，ip，每日1次，每周6次，共4周的剂量下，对人肝癌(Bel-7402)裸鼠移植瘤的抑瘤率分别为32.0%，50.5%和60.2%；在上述3个剂量下，对人肺腺癌(GLC-82)裸鼠移植瘤的抑瘤率分别为30.3%，41.9%和56.1%。结果表明，阿诺宁乳化剂ig，ip对上述5种肿瘤均有明显的抑制作用，且其抑瘤率与阿诺宁乳剂的剂量相关。用Bliss方法统计出阿诺宁乳化剂对小鼠ig一次的半数致死量(LD₅₀)及其95%可信限为74.75 (58.67～5.26) mg·kg^-1；ip的LD₅₀)为1.12 (1.01～1.24)mg·kg^-1)，静脉注射(iv)的LD₅₀为1.81(1.61～2.03) mg·kg^-1。结论阿诺宁乳化剂对多种小鼠和裸鼠移植瘤均有明显抗瘤作用。

Studies on antitumor effects and acute toxicity of anuoning emulsion

OBJECTIVE To investigate the antitumor effect and acute toxicity of anuoning emulsion in mice. METHODS The models transplanted tumor in mice and nude mice were used.Blliss method was used to calculate the lethal dose of 50% (LD₅₀) of anuoning emulsion in mice. RESULTS Under the doses of 4,8 and 16 mg·kg^-1 anuoning emulsion intragstic(ig),qd×10 d,the average tumor inhibition rates were 39.2%,46.9% and 55.7% respectively (P＜0.01),against mice tumor HepS.The average tumor inhibition rates were 34.6%,47.3% and 54.4% (P＜0.01) against S-180 sarcoma in mice.In L₂tumor,the mean inhibition rates were 37.5%,45.9%,and 56.5% (P＜0.05～0.01),respectively.Under the doses of anuoning emulsion 7.5,15,30 and 60 μg·kg^-1,intraperitoneal injection (ip) qd×10 d to mice tumor HepS,the mean inhibition rates were 31.0%,42.9%,50.2% and 62.4%(P＜0.05～0.01),respectively.But In S-180 sarcoma the mean inhibition rates were 23.6%,39.4%,50.9% and 61.2% (P＜0.05～0.01),respectively.Under the doses of anuoning emulsion 15，30 and 60μg·kg^-1,ip×6/w×4 w,the inhibitory rates were 32.0%,50.5% and 60.2% (P＜0.01) against xenograft tumor of human liver carcinoma (Bel-7402) in nude mice.Under the same doses,ip×6/w×3 w,the inhibition rates were 30.3%,41.9% and 56.1%,respectively,against xenograft tumor of human lung adenocarcinoma (GLC-82) in nude mice.The results showed that anuoning emulsion possessed antitumor effect against above mentioned 5 tumors in mice and nude mice after ip and ig administration.In single intragastic (ig) management to mice,LD₅₀ and its 95% confidence interval was 74.75 (58.669～95.26) mg·kg⁵⁰.In single ip management to mice,LD₅₀of anuoning emulsion was 1.12(1.01～1.24) mg·kg^-1.In single intravenous (iv) administration LD₅₀ was 1.81 (1.61～2.02) mg·kg^-1. CONCLUSION The experiment results indicated that anuoning emulsion possessed obvious antitumor effects against various transplanted tumor in mice and nude mice.