知母总皂苷对大鼠脑缺血及再灌注损伤的保护作用

 目的观察知母总皂苷(SAaB)对于大鼠脑缺血及再灌注损伤的保护作用。方法大鼠灌胃给药7d,采用双侧颈总动脉结扎法制备大鼠急性全脑缺血模型,测定脑组织含水量,制备脑匀浆测定脑超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量；采用大脑中动脉栓塞法制作大鼠急性局灶缺血模型(MCAO),观察大鼠神经功能损害改变并评分,脑组织用氯化三苯基四氮唑(TTC)染色测定脑梗死容积。结果SAaB低、高剂量组(50,100 mg·kg^-1)均能降低缺血损伤后脑组织含水量、增加缺血组织SOD活性,降低异常MDA含量；同时MCAO模型中SAaB各剂量组大鼠缺血再灌注后神经功能损害评分均低于对照组,其缺血侧脑组织梗死体积占半侧脑组织的百分比亦较对照模型组降低。所有组间差异具有统计学意义(P＜0.05)。结论SAaB对于脑缺血再灌注后引起的脑损伤具有一定的保护作用。

Protective Effect of Timosaponin on Cerebral Ischemia-Reperfusion in Rats

OBJECTIVE To study the protective effect of saponins from Anemarrhena asphodeloides Bge(SAaB)on cerebral ischemia- reperfusion injury in rats.METHODS Before the ischemia occurred,the SAaB and saline were garaged to the rats via gastric and lasted for seven days.Then the model of acute reperfusion injury after cerebral ischemia was made by two means:bilateral carotid arteries were clamped in one group,the middle cerebral artery occlusion(MCAO)was jammed in the other group.After the ischemic condition lasted for 2 hours following a 22 hours reperfusion,the behavior grade of cerebral apoplexy was evaluated,water content in ischemia areas was measured,the changes of su- peroxide dismutase(SOD)activity and malonaldehyde(MDA)content were determined.The infarction area of the brain was stained by 2% TTC.RESULTS After statistical analysis,SAaB significantly decreased the content of the brain water and MDA in isehemia areas,increased SOD activity.Besides,the behavior grade of cerebral apoplexy and infarction areas of the brain after being stained by TTC in these two SAaB groups were much lower than those in control group(all P＜0.01).CONCLUSION SAaB has definitive cerebral protective effects against is- chemia-reperfusion injury.