人工骨异烟肼体外及家兔体内释放度研究

 目的：了解蜂蜡为阻滞剂包裹的异烟肼-人工骨(INH-PHA-wax)与未包裹蜂蜡的异烟肼-人工骨(INH-PHA)体外及家兔体内的释放度。方法：用RP-HPLC在不同时间分别测定体外及植入家兔体内的包裹或未包裹蜂蜡的异烟肼-人工骨中异烟肼的浓度。结果：包裹15%蜂蜡INH-PHA-wax,体外20 d释药66%;包裹25%蜂蜡INH-PHA-wax,20 d释药22%;未包裹蜂蜡INH-PHA,6 d释药97%。包裹25%蜂蜡INH-PHA-wax植入家兔体内，可持续释药达240 d,而未包裹蜂蜡INH-PHA持续释药仅60 d。结论：INH-PHA-wax可有效地控制药物的释放，对治疗骨结核可能是一有效植入式缓释剂。

Dissolution of isonizide in isonizide-hydroxylapatite ceramic composites in vitro and in rabbits

OBJECTIVE:To study the dissolution of isonizide(INH)in isonizide-hydroxylapatite ceramic composites (INH-PHA)encapsulated with the wax as a ratardant(INH-PHA-wax)and INH-PHA without the wax in vitro and in rabbits.METHOD:The concentration of INHin vitro and vivo at different times was determined by reversed HPLC respectively.RESULTS:66%of INH in INH-PHA-wax encapsulated with 1 5%wax was released in vitro at the 20th day,22%in INH-PHA-wax with 25%wax at the 20th day,97%in INH-PHA(without wax)at the sixth day. Releasing of INH maintained for 240 days in INH-PHA-wax encapsulated with 25%wax in rabbits,but only for 60 days in INH-PHA without wax.CONCLUSION: Releasing of INH could be controlled with INHPHA-wax.It may be an effective implanting sustained biomaterial to treat bone tuberculosis.