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l-四氢巴马汀对羟考酮依赖大鼠神经元损伤的保护作用
引用本文:刘艳丽,颜玲娣,周培岚,吴春福,宫泽辉.l-四氢巴马汀对羟考酮依赖大鼠神经元损伤的保护作用[J].中国药学杂志,2009,44(1):32-35.
作者姓名:刘艳丽  颜玲娣  周培岚  吴春福  宫泽辉
作者单位:1.苏州大学药学院 江苏苏州 215123;2.军事医学科学院毒物药物研究所 北京 100850;3.沈阳药科大学药理教研室 沈阳 110016
基金项目:解放军总后“十五”指令性课题(01L038)
摘    要: 目的探讨羟考酮依赖引起大鼠海马CA1区和中脑腹侧被盖区(VTA)内神经元的损伤情况,以及l-四氢巴马汀(l-THP)对羟考酮依赖大鼠神经元损伤的保护作用。方法采用连续递增给羟考酮建立依赖模型:大鼠连续给药22 d,每天2次,从第1天到第8天,从开始剂量2 mg·kg–1,每日递增1 mg至第8天固定为9 mg·kg–1,直到第22天。l-THP伴随给药,l-THP灌胃给药40 min后给羟考酮。末次给药24 h后,取脑,石蜡切片,采用ABC法测海马CA1区和VTA内GFAP的免疫组织化学反应,以其阳性神经元的平均吸光度值表示其含量。结果羟考酮引起大鼠海马CA1区和VTA内GFAP含量明显升高,提示羟考酮能造成神经元损伤。l-THP(15,30 mg·kg–1)抑制海马CA1区和VTA内GFAP含量的升高。结论长期大剂量给予羟考酮能造成大鼠神经元损伤,l-THP对羟考酮依赖大鼠神经元损伤有保护作用。

关 键 词:l-四氢巴马汀  羟考酮  神经胶质纤维酸性蛋白
收稿时间:2008-01-03;

Protection of l-Tetrahydropalmatine on Oxycodone Induced Neronal Damage in Rats
LIU Yan-li,YAN Ling-di,ZHOU Pei-lan,WU Chun-fu,GONG Ze-hui.Protection of l-Tetrahydropalmatine on Oxycodone Induced Neronal Damage in Rats[J].Chinese Pharmaceutical Journal,2009,44(1):32-35.
Authors:LIU Yan-li    YAN Ling-di  ZHOU Pei-lan  WU Chun-fu  GONG Ze-hui
Institution:1. College of Pharmacy,Soochow University,Suzhou 215123,China;2. Beijing Institute of Pharmacology and Toxicology,Beijing 100850,China;3. Department of Pharmacology,Shenyang Pharmaceutical University,Shenyang 110016,China
Abstract:OBJECTIVE To investage effects of l-tetrahydropalmatine on neuron damage induced by oxycodone in rats.METHODS Rats were administered for 22 consecutive days with oxycodone,twice every day.Oxycodone was increased progressively at the oringinal dose of 2 mg·kg–1 frome 1st day to 8th day,then,given at the dose of 9 mg·kg–1 from 8th day to 22nd day.l-THP was administrated 40 min before treatment of oxycodone.The levels of glial fibrillary acidic protein(GFAP) were determined as immunohistochemical intensity.RESULTS Content of GFAP induced by oxycodone was increased in the CA1and VTA or NAc region.l-THP decreased content of GFAP in the CA1 or VTA region.CONCLUSION Impairment of neron was induced by oxycodone in rats.l-THP could inhibit the damage,which suggested that l-THP might be benefit for clinical use to prevent oxycodone-dependence.
Keywords:l-tetrahydropalmatine" target="_blank">l-tetrahydropalmatine')" href="#">l-tetrahydropalmatine  oxycodone  GFAP
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