炒紫苏子提取物的抗氧化作用研究

 目的　研究炒紫苏子提取物的抗氧化作用。方法　15月龄昆明种小鼠,随机分组,每日1次灌胃炒紫苏子醇提药物和水提药物,给药21次后,杀鼠取脑和血。采用MDA试剂盒、SOD试剂盒,测定血浆中MDA,SOD值,采用紫外分光光度法测小鼠脑匀浆中MAO。结果 炒紫苏子醇提物269,134 mg·kg^-1剂量组均能显著降低MDA水平(P<0.001),提高SOD酶活性(P<0.05),而134 mg·kg^-1剂量组能显著降低MAO水平(P<0.05);炒紫苏子水提物213 mg·kg^-1剂量组均能显著降低MDA,MAO水平(P<0.001,P<0.01),提高SOD酶活性(P<0.05),而106 mg·kg^-1剂量组能显著降低MDA水平(P<0.001),MAO水平(P(0.05)。结论 炒紫苏子醇提取物和水提取物具有较强的抗氧化作用。

Study on the antioxidative effect of roasted perilla seed

OBJECTIVE To study the antioxidative effect of the extracts from roasted perilla seed (RPS) .METHODS 15 month-old mice of Kunming species were divided into groups at random. After the mice were orally treated with the RPS extracts once daily for 21 days, blood and brains were collected for determining MDA,SOD and MAO levels in plasma or in the brain homogenate respectively.RESULTS The MDA level was reduced significantly in the group treated with 269 mg·kg^-1(P<0.001) and 134mg·kg^-1 (P<0.05) ethanol extract of RPS. The MAO level also was reduced in the group treated with 134 mg·kg^-1 ethanol extract of RPS (P<0.05). The MDA and MAO level was decreased significantly (P<0.001,P<0.01), and the activity of SOD was enhanced (P<0.05) in the group treated with 213 mg·kg^-1 aqueous extract of RPS. The MDA and MAO level was reduced (P<0.001,P<0.01) in the groups treated with 106 mg·kg^-1 aqueous extract of RPS.CONCLUSION The ethanol extract and aqueous extract of RPS enhanced the antioxidative ability of aged mice.