电化学法研究枸杞子及黄精对活性氧自由基的清除作用

 目的:研究枸杞子和黄精对活性氧自由基(O^-·₂和·OH)的清除作用。方法:通过邻苯三酚在碱性环境下自氧化产生O^-·₂,Fenton体系产生·OH,自旋捕集剂PBN捕捉产生的·OH。单扫示波极谱法检测枸杞子和黄精对O^-·₂和·OH的抑制作用。结果:枸杞子和黄精对O^-·₂和·OH具有明显的抑制作用。分别计算了它们的IC₅₀值。枸杞子对O^-·₂和·OH的清除能力为:同仁堂宁夏枸杞>市售宁夏枸杞>河北枸杞>内蒙枸杞。黄精对O^-·₂和·OH的清除效能力:酒制黄精>蜜炙黄精。结论:枸杞子和黄精都能有效地清除活性氧自由基(O^-·₂,·OH)具有抗氧化作用,可能是它们所具有的抗氧化等药理作用机理之一。

Scavenging effect of fructus lycii and rhizoma polygonati on active oxygen free radicals

OBJECTIVE:The scavenging effect of Fructus Lycii and Rhizoma Polygonati on active oxygen free radicals(O^-·₂and·OH)was investigated.METHOD:The superoxide anion radical was produced by the autoxidation of pyrogallol in alkaline solution,and the hydroxyl radical was generated from the Fenton system.Phenyl-tert-butylnitrone(PBN)spin trap was used to trap the hydroxyl radical.Single sweep oscillopolarography was applied to detect the generation of O^{- ·}₂ and·OH.RESULTS:Fructus Lycii and Rhizoma Polygonati had obvious inhibitory effectonO^-·₂and·OH.The irIC₅₀ values were calculated respectively.The scavenging ability of Fructus LyciionO^-·₂and·OH was:Tongrentang Fructus Lycii barbari>than on market>Hebei Fructus Lycii>that of NeiMongol Fructus lycii.The scavenging effect of Rhizoma PolygonationO^-·₂and ·OH was polygonatum mix-fried with winewas stronger than that of polygonatum mix-fried with honey.CONCLUSION :Fructus Lyciiand Rhizoma Polygonati could scavenge the active oxygen radicals(O^-·₂>^-·₂,·OH)effectively,and they had antioxidative effect.The scavenging effect on free radicals of Fructus Lycii and Rhizoma Polygonati was probably part of the irpharmacological mechanism.