库拉索芦荟多糖对小鼠腹腔巨噬细胞的体外激活作用

 目的　研究库拉索芦荟多糖对小鼠腹腔巨噬细胞的激活作用。方法　用25,50,100,200,400μg·mL^-1的库拉索芦荟多糖体外诱导小鼠腹腔巨噬细胞48h ,测定细胞内乳酸脱氢酶、酸性磷酸酶、精氨酸酶活性及释放的TNF-α和IL-1含量。结果　库拉索芦荟多糖能上调腹腔巨噬细胞内乳酸脱氢酶、酸性磷酸酶和精氨酸酶活性,并能显著诱导腹腔巨噬细胞表达TNF-α和IL-1,增强吞噬中性红能力。结论　库拉索芦荟多糖对小鼠腹腔巨噬细胞具有激活作用。

Activation effects of polysaccharide from Aloe barbadensis on mouse peritoneal macrophages in vitro

OBJECTIVE To study the activation effects of polysaccharide from Aloe barbadensis Milller on mouse peritoneal macrophages. METHODS After the mouse peritoneal macrophages were cultured with 25,50,100,200,400μg·mL^-1 polysaccharide from Aloe barbadensis Milller for 48h in vitro, the activities of lactate dehydrogenase (LDH), acid phosphatase (ACP) and arginase (ARG) in the macrophages were detected. And the expression levels of tumor necrosis factor alpha (TNF-α)and interleukin-1 (IL-1) were analyzed.RESULTS The activities of LDH, ACP and ARG in macrophages were increased by polysaccharide from Aloe barbadensis Milller. And significantly more expression of TNF-α and IL-1 were produced. The phagocytic capacity of macrophage in the treatment group was stronger than that of the control.CONCLUSION The polysaccharide from Aloe barbadensis Milller can activate the mouse peritoneal macrophages.