6种中药活性成分植物雌激素作用的比较研究

 目的观察蜕皮甾酮等6种中药活性成分对MCF-7细胞增殖、细胞周期的影响,以探讨它们的植物雌激素样作用及其可能机制。方法采用MTT细胞增殖实验观察6种中药活性成分:蜕皮甾酮、红花黄色素A、丹参酮I、丹参酮ⅡA、补骨脂素、异补骨脂素对雌激素受体(ER)阳性细胞系MCF-7增殖的影响,并以雌激素受体拮抗剂IC I182,780干预,探讨其发挥雌激素样作用的可能机制。同时,通过流式细胞术检测这些活性成分对MCF-7细胞增殖周期的影响。结果蜕皮甾酮、红花黄色素A、补骨脂素、异补骨脂素能够促进ER阳性细胞系MCF-7增殖,并将细胞周期由G1期向S期推进,促进DNA合成,提高细胞分裂增殖指数。同时,加入ER拮抗剂IC I182,780可抑制其促增殖作用。丹参酮I和丹参酮ⅡA能够抑制ER阳性细胞系MCF-7增殖,但对其细胞周期影响不显著。同时,加入ER拮抗剂IC I182,780可减弱其对MCF-7增殖的抑制作用。结论蜕皮甾酮、红花黄色素A、补骨脂素、异补骨脂素能够促进MCF-7增殖,而丹参酮I和丹参酮ⅡA可抑制MCF-7细胞增殖,其作用均是通过ER介导的。

Phytoestrogenic Effects of Six Active Components in Chinese Medicine

OBJECTIVE To evaluate the estrogenic effects and their mechanisms of six active components in Chinese medicine through the test of their influence on MCF-7 proliferation and its cell cycle.METHODS The proliferation of MCF-7 influenced by ecdysterone,saffcomin A,tanshinone I,tanshinone ⅡA,psoralen and isopsoralen was analyzed by MTT assay and their mechanisms were studied by adding ER antagonist ICI180,782.The cell cycle distribution of MCF-7 was determined by flow cytometry.RESULTS The proliferation rates of the cells treated with ecdysterone,saffcomin A,psoralen and isopsoralen were markedly increased.While the cell cycle was impulsed from G1 to S.DNA synthesis was inhanced and PI was increased.The above function on boosting proliferation was inhibited by adding estrogen receptor antagonism ICI182,780.Tanshinone I and tanshinone ⅡA showed inhibitory effects on proliferation of ER positive MCF-7 cell.However,no distinct effects were obtained on cell cycle.At the same time,the inhibitory effects of tanshinone I and tanshinone ⅡA were significantly decreased by adding the estrogen receptor antagonist ICI182,780.CONCLUSION Ecdysterone,saffcomin A,psoralen and isopsoralen can improve MCF-7 proliferation.While tanshinone I and tanshinone ⅡA have inhibitory effects on MCF-7 proliferation.All of their effects were realized through estrogen receptor.