知母宁对豚鼠哮喘的预防作用及对体内一氧化氮和内皮素的影响

 目的：观察知母宁对豚鼠哮喘发作的预防作用并探讨部分机制。方法：将67只豚鼠随机分为：正常对照组；哮喘组；知母宁组；地塞米松组及知母宁＋地塞米松组。观察肺／体重比值；诱喘时间；哮喘持续时间；血清及肺泡灌洗液（BALF）中一氧化氮（NO）和内皮素-1（ET-1）水平的变化。结果：①知母宁组豚鼠诱喘时间及哮喘持续时间均较哮喘组明显延长；抽搐发生率明显低于哮喘组；肺组织炎性反应明显减轻，与地塞米松组接近。②知母宁组血清NO及BALF中ET-1水平均较哮喘组显著下降，也与地塞米松组相似。结论：知母宁有与肾上腺糖皮质激素相似的预防哮喘发作的作用。可能部分通过减少NO和ET-1生成而起作用。

The preventing effect of chimonin on guinea pigs asthma and its interfering action on the level of nitric oxide and endothelin in the body

OBJECTIVE:To observe the preventing effect of chimonin on guinea pigs asthma and explore its partly action mechanism.METHODS:67 guinea pigs were divided into five groups at randomicy:the groups of normal control,asthma,chimonin,dexamethasone and chimonin with dexamethasone.The ratio of wet lung weight to body weight,the period needed for inducing asthma attack and the sustained period of asthma episode were observed.The concentration of nitric oxide(NO) and endothelin-1(ET-1) in serum and bronchoalveolar lavage fluid(BALF) were detected respectively.RESULTS:① the inducing and sustained period of asthma episode in group chimonin were distinctly longer than those in group asthma;the occurrence rate of spasm in group chimonin was evidently lower than group asthma;the inflammatory reaction of the lung tissues in group chimonin relieved obviously and this change was similar to the group dexamethasone.② The NO in serum and ET-1 of BALF in group chimonin were lower than group asthma and similar to the group dexamethasone.CONCLUSION:chimonin can partly prevent asthma episode and this role is similar to glucocorticoid.It plays the role partly by reducing the level of NO and ET-1 in the body.